This research aimed to apply the acidification method to remove impurities from biodiesel-derived crude glycerol (BCG) and investigate the potential of purified BCG (PBCG) to be used as a carbon source for microbial oil production by oleaginous yeast Pseudozyma parantarctica CHC28. The optimum purifying conditions of BCG were pretreated with hydrochloric acid at a pH of 1.71. The PBCG concentration was 469.52 g/L under optimized conditions. The batch fermentation was studied using a nitrogen-limited medium containing PBCG (PBCG-medium). The PBCG-medium with 50 g of PBCG per liter provided the maximum biomass and oil concentration of 11.10 g/L and 4.07 g/L, respectively. The oil accumulation was approximately 45%. The long-chain fatty acids C16–C18 were the main compositions, which accounted for over 85%. These results suggested that the conversion of PBCG into microbial oil was an interesting direction to produce microbial oil. It was also a remarkable solution to add value to the by-products from biodiesel production. The mathematical model was demonstrated to describe satisfactorily the yeast growth and microbial oil production profile achieved in media containing pure glycerol and PBCG as carbon sources.

本研究旨在应用酸化方法去除生物柴油衍生的粗甘油 (BCG) 中的杂质，并研究纯化的 BCG (PBCG) 作为产油酵母Pseudozyma parantarctica生产微生物油的碳源的潜力CHC28。BCG的最佳纯化条件是用pH为1.71的盐酸进行预处理。优化条件下PBCG浓度为469.52 g/L。使用含 PBCG 的限氮培养基（PBCG 培养基）研究分批发酵。每升含 50 g PBCG 的 PBCG 培养基提供的最大生物量和油浓度分别为 11.10 g/L 和 4.07 g/L。油积累为约45%。长链脂肪酸C16-C18是主要成分，占85%以上。这些结果表明，将PBCG转化为微生物油是生产微生物油的一个有趣的方向。这也是增加生物柴油生产副产品价值的非凡解决方案。