A taxonomic investigation using a polyphasic method was conducted to identify a novel marine flavobacterium, designated as DJ-13^T, isolated from the hard coral Acropora sp. collected at Okinawa, Japan. Bacterial cells were Gram-stain-negative, yellow-colored, strictly aerobic, rod-shaped, catalase- and oxidase-positive, non-motile, and chemoorganoheterotrophic. The novel isolate grew at NaCl concentrations of 0.5–7%, pH 6.5–9.0, and 15–37 °C. A phylogenetic study on the basis of the 16S rRNA gene sequence revealed that strain DJ-13^T belongs to the family Flavobacteriaceae and that it shared the greatest sequence similarity (95.9%) with Croceivirga lutea CSW06^T. Strain DJ-13^T comprised iso-C17:0 3-OH, iso-C15:0, and iso-C15:1 G as the main (> 10%) cellular fatty acids. Menaquinone-6 (MK-6) was the only respiratory quinone. The assembled draft genome size of strain DJ-13^T was 3.71 Mbp with G + C content of 38.7 mol%. The average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH), and average amino acid identity (AAI) values of DJ-13^T and the species of the genus Croceivirga were found to be 74.9–75.5%, 13.4–14.7%, and 68.2–72.4%, respectively. Strain DJ-13^T contained phosphatidylethanolamine, two unidentified aminolipids, and five unidentified lipids as polar lipids. From the polyphasic taxonomic results presented, the strain is considered to represent a novel species of the genus Croceivirga for which the name Croceivirga thetidis sp. nov. is proposed. The type strain of C. thetidis sp. nov. is DJ-13^T (= KCTC 72790^T = NBRC 114252^T).

使用多相方法进行分类学研究，以鉴定一种新的海洋黄杆菌，命名为 DJ-13 ^T，从硬珊瑚Acropora sp. 中分离出来。采集于日本冲绳。细菌细胞为革兰氏染色阴性、黄色、严格需氧、杆状、过氧化氢酶和氧化酶阳性、非运动性和化学有机异养的。新型分离物在 NaCl 浓度为 0.5-7%、pH 值 6.5-9.0 和 15-37 °C 下生长。基于 16S rRNA 基因序列的系统发育研究表明，菌株 DJ-13 ^T属于黄杆菌科，与Croceivirga lutea CSW06 ^T具有最大的序列相似性（95.9%）。株 DJ-13^T包括作为主要 (> 10%) 细胞脂肪酸的异 C17:0 3-OH、异 C15:0 和异 C15:1 G。Menaquinone-6 (MK-6) 是唯一的呼吸醌。菌株 DJ-13 ^T的组装基因组草图大小为 3.71 Mbp，G + C 含量为 38.7 mol%。发现 DJ-13 ^T和Croceivirga属物种的平均核苷酸同一性 (ANI)、数字 DNA-DNA 杂交 ( d DDH) 和平均氨基酸同一性 (AAI) 值为74.9-75.5%、13.4-分别为 14.7% 和 68.2-72.4%。应变 DJ-13 ^T含有磷脂酰乙醇胺、两种未知的氨基脂质和五种未知的脂质作为极性脂质。从所提供的多相分类学结果来看，该菌株被认为代表了Croceivirga属的一个新物种，其名称为Croceivirga thetidis sp。十一月 被提议。C. thetidis sp.的类型菌株。十一月 是 DJ-13 ^T (= KCTC 72790 ^T  = NBRC 114252 ^T )。