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Identification and classification of Croceivirga thetidis sp. nov., a marine Flavobacteriaceae isolated from the hard coral Acropora

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Abstract

A taxonomic investigation using a polyphasic method was conducted to identify a novel marine flavobacterium, designated as DJ-13T, isolated from the hard coral Acropora sp. collected at Okinawa, Japan. Bacterial cells were Gram-stain-negative, yellow-colored, strictly aerobic, rod-shaped, catalase- and oxidase-positive, non-motile, and chemoorganoheterotrophic. The novel isolate grew at NaCl concentrations of 0.5–7%, pH 6.5–9.0, and 15–37 °C. A phylogenetic study on the basis of the 16S rRNA gene sequence revealed that strain DJ-13T belongs to the family Flavobacteriaceae and that it shared the greatest sequence similarity (95.9%) with Croceivirga lutea CSW06T. Strain DJ-13T comprised iso-C17:0 3-OH, iso-C15:0, and iso-C15:1 G as the main (> 10%) cellular fatty acids. Menaquinone-6 (MK-6) was the only respiratory quinone. The assembled draft genome size of strain DJ-13T was 3.71 Mbp with G + C content of 38.7 mol%. The average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH), and average amino acid identity (AAI) values of DJ-13T and the species of the genus Croceivirga were found to be 74.9–75.5%, 13.4–14.7%, and 68.2–72.4%, respectively. Strain DJ-13T contained phosphatidylethanolamine, two unidentified aminolipids, and five unidentified lipids as polar lipids. From the polyphasic taxonomic results presented, the strain is considered to represent a novel species of the genus Croceivirga for which the name Croceivirga thetidis sp. nov. is proposed. The type strain of C. thetidis sp. nov. is DJ-13T (= KCTC 72790T = NBRC 114252T).

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Acknowledgements

This research was supported by the Bisa Research Grant of Keimyung University in 2020.

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Author notes

  1. Mina Yasumoto-Hirose

    Present address: Tropical Technology Plus, 12-75 Suzaki, Uruma, Okinawa, 904-2234, Japan

Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea

    Jaewoo Yoon

  2. Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, , Iwate, 026-0001, Japan

    Mina Yasumoto-Hirose

  3. Sanriku Education and Research Center for Marine Biosciences, Kitasato University School of Marine Biosciences, 160-4 Utou, Okirai, Sanriku-cho, Ofunato, Iwate, 022-0101, Japan

    Hiroaki Kasai

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  1. Jaewoo Yoon
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  3. Hiroaki Kasai
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Contributions

Conceived and designed the experiments: JY. Performed the experiments: JY, MYH, HK. Analyzed the data: JY, HK. Wrote the paper: JY.

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Correspondence to Jaewoo Yoon.

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10482_2021_1611_MOESM1_ESM.pdf

Supplementary Fig. 1 A two-dimensional thin-layer chromatogram showing the total polar lipid compositions of DJ-13T and reference strains. Total polar lipids were detected by spraying the plate with molybdatophosphoric acid. PE: phosphatidylethanolamine, AL: unidentified aminolipid, L: unidentified lipid

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Yoon, J., Yasumoto-Hirose, M. & Kasai, H. Identification and classification of Croceivirga thetidis sp. nov., a marine Flavobacteriaceae isolated from the hard coral Acropora. Antonie van Leeuwenhoek 114, 1407–1416 (2021). https://doi.org/10.1007/s10482-021-01611-w

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