Toxic heavy metals pollution posed severe health hazards to the environment and biodiversity. Therefore, the development of rapid and non-invasive bioassays is in the race to monitor toxic chemicals using novel approaches. This study isolated and characterized an intense blue luminescence-producing marine bacteria, Vibrio campbellii STF1, for biosensing applications. Species-level identification of this strain was confirmed based on various phenotypic tests and multilocus sequence approach using 16s rRNA, toxR, and luxA gene sequence analysis. Fatty acid methyl ester analysis revealed the presence of three predominant fatty acids C_{15:0 anteiso} (21.73%), C_{17:0 anteiso} (11.27%), and C_{19:0 anteiso} (9.08%) in STF1. Luciferase enzyme from V. campbellii STF1 was extracted, partially purified, and molecular masses (alpha subunit 40 kDa and beta subunit 37 kDa) were determined by SDS-PAGE gel for in vivo assays. MALDI-TOF-MS analysis of V. campbellii cells’ protein extracts showed distinct mass spectral peaks at m/z of 2615, 3948, and 4232 da. V. campbellii STF1 is resistant to heavy metal lead, while other metals such as cadmium, copper, and mercury inhibited its growth and luminescence. Crude ethyl acetate extraction of V. campbellii demonstrated antibacterial activity against Shigella dysenteriae type 5 with a maximum inhibition zone of 27.0±1.0 mm.

有毒重金属污染对环境和生物多样性造成严重的健康危害。因此，快速和非侵入性生物测定的发展正在竞相使用新方法监测有毒化学物质。这项研究分离并表征了一种产生强烈蓝色发光的海洋细菌Vibrio campbellii STF1，用于生物传感应用。该菌株的物种水平鉴定基于各种表型测试和使用 16s rRNA、toxR 和 luxA 基因序列分析的多位点序列方法得到确认。脂肪酸甲酯分析显示存在三种主要脂肪酸 C _{15:0 反异}(21.73%)、C _{17:0 反异}(11.27%) 和 C _{19:0 反异}(9.08%) 在 STF1 中。提取、部分纯化来自V. campbellii STF1 的荧光素酶，并通过用于体内测定的 SDS-PAGE 凝胶测定分子量（α 亚基 40 kDa 和 β 亚基 37 kDa）。V. campbellii细胞蛋白质提取物的 MALDI-TOF-MS 分析显示在 m/z 为 2615、3948 和 4232 da 处有明显的质谱峰。V. campbellii STF1 对重金属铅具有抵抗力，而镉、铜和汞等其他金属抑制其生长和发光。V. campbellii的粗乙酸乙酯提取物对 5 型痢疾杆菌具有抗菌活性，最大抑菌圈为 27.0±1.0 mm。