The plant-specific transcription factors NACs (NAM, ATAF1, 2, CUC), one major transcription factor family, play significant roles in various physiological processes including abiotic stresses. In our study, an NAC gene from potato (Solanum tuberosum L.) was cloned and named as StNAC1 for a high-sequence similarity to SlNAC1, a well-known tomato NAC gene regulating multiple stress responses and fruit ripening. StNAC1 gene was significantly induced under salt stress. Then, we constructed StNAC1-overexpressing transgenic Nicotiana benthamiana plants and obtained three homozygous transgenic lines. The phenotypic analysis results showed that StNAC1-overexpressing transgenic plants had not only higher seed germination and green leaf rates, but also accumulated less ROS and more proline than wide-type plants under salt stress, which resulted in improving transgenic plants salt tolerance. These suggested that StNAC1 gene might function as a positive regulator in plant response to salt stress.

植物特异性转录因子 NAC（NAM、ATAF1、2、CUC）是一个主要的转录因子家族，在包括非生物胁迫在内的各种生理过程中发挥着重要作用。在我们的研究中，来自马铃薯 ( Solanum tuberosum L.)的NAC基因被克隆并命名为StNAC1，因为它与SlNAC1具有高序列相似性，SlNAC1是一种众所周知的调节多重应激反应和果实成熟的番茄NAC基因。StNAC1基因在盐胁迫下显着诱导。然后，我们构建了过表达StNAC1 的转基因本氏烟草植物并获得三个纯合转基因品系。表型分析结果表明，与盐胁迫下的宽型植物相比，过表达StNAC1的转基因植物不仅具有更高的种子发芽率和绿叶率，而且积累了更少的ROS和更多的脯氨酸，从而提高了转基因植物的耐盐性。这表明StNAC1基因可能在植物对盐胁迫的反应中起正调节作用。