The present investigation primarily focussed on evaluating the efficacy of exogenous proline on the flower longevity of Dianthus chinensis L. Floral buds were harvested at the paint brush stage (i.e., a day prior to anthesis) and divided into 6 sets, with one set of buds (i.e., control) held in distilled water and rest of the 5 sets were supplemented with various concentrations of proline, viz., 10 mM, 20 mM, 30 mM, 40 mM and 50 mM. The application of proline at 40 mM concentration proved out to be most effective in improving the longevity of the flowers by about 4 days as compared to the control. The ameliorated longevity coincided with enhanced floral diameter, fresh mass, dry mass and water content. The flowers with delayed senescence also maintained higher soluble proteins, sugars and phenols. The results suggest that exogenous proline effectively alleviates oxidative stress in the petal tissue, as evident by a relatively lower maloendialdehyde content, which is manifested in the form of reduced lipid peroxidation (LPO). Reduced LPO was commensurate with increased membrane stability, quantified by membrane stability index. Moreover, the flowers with improved longevity exhibited a decline in lipoxygenase activity and significant augmentation of antioxidant enzymes superoxide dismutase, catalase and ascorbate peroxidase.

本研究主要集中在评价外源脯氨酸对石竹花寿命的影响。L. 在刷漆阶段（即开花前一天）收获花蕾并分成6组，其中一组花蕾（即对照）置于蒸馏水中，其余5组补充各种脯氨酸的浓度，即 10 mM、20 mM、30 mM、40 mM 和 50 mM。事实证明，与对照相比，应用 40 mM 浓度的脯氨酸最有效地将花的寿命延长约 4 天。延长的寿命与增加的花直径、新鲜质量、干质量和含水量相吻合。延迟衰老的花还保持较高的可溶性蛋白质、糖和酚类物质。结果表明，外源脯氨酸有效地缓解了花瓣组织中的氧化应激，丙二醛含量相对较低就证明了这一点，其表现为减少的脂质过氧化（LPO）。减少的 LPO 与增加的膜稳定性相称，由膜稳定性指数量化。此外，长寿花表现出脂氧合酶活性下降和抗氧化酶超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶显着增加。