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Is proline the quintessential sentinel of plants? A case study of postharvest flower senescence in Dianthus chinensis L.

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Abstract

The present investigation primarily focussed on evaluating the efficacy of exogenous proline on the flower longevity of Dianthus chinensis L. Floral buds were harvested at the paint brush stage (i.e., a day prior to anthesis) and divided into 6 sets, with one set of buds (i.e., control) held in distilled water and rest of the 5 sets were supplemented with various concentrations of proline, viz., 10 mM, 20 mM, 30 mM, 40 mM and 50 mM. The application of proline at 40 mM concentration proved out to be most effective in improving the longevity of the flowers by about 4 days as compared to the control. The ameliorated longevity coincided with enhanced floral diameter, fresh mass, dry mass and water content. The flowers with delayed senescence also maintained higher soluble proteins, sugars and phenols. The results suggest that exogenous proline effectively alleviates oxidative stress in the petal tissue, as evident by a relatively lower maloendialdehyde content, which is manifested in the form of reduced lipid peroxidation (LPO). Reduced LPO was commensurate with increased membrane stability, quantified by membrane stability index. Moreover, the flowers with improved longevity exhibited a decline in lipoxygenase activity and significant augmentation of antioxidant enzymes superoxide dismutase, catalase and ascorbate peroxidase.

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References

  • Abdelaal KAA, Attia KA, Alamery SF, El-Afry MM, Ghazy AI, Tantawy DS, Al-Doss AA, El-Shawy ESE, Abu-Elsaoud AM, Hafez YM (2020) Exogenous application of proline and salicylic acid can mitigate the injurious impacts of drought stress on barley plants associated with physiological and histological characters. Sustain 12:1736

    Article  CAS  Google Scholar 

  • Aebi H (1984) Catalase in Vitro. Meth Enzymol 105:121–126

    Article  CAS  Google Scholar 

  • Ahmad SS, Tahir I (2016a) How and why of flower senescence: understanding from models to ornamentals. Indian J Plant Physiol 21:446–456

    Article  Google Scholar 

  • Ahmad SS, Tahir I (2016b) Increased oxidative stress, lipid peroxidation and protein degradation trigger senescence in Iris versicolor L. flowers. Physiol Mol Biol Plants 22:507–514

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ahmad SS, Tahir I (2017) Regulatory role of phenols in flower development and senescence in the genus Iris. Indian J Plant Physiol 22:135–140

    Article  Google Scholar 

  • Ahmed CB, Magdich S, Rouina BB, Sensoy S, Boukhris M, Abdullah FB (2011) Exogenous proline effects on water relations ions contents in leaves and roots of young olive. Amino Acids 40:565–573

    Article  PubMed  CAS  Google Scholar 

  • AlKahtani MD, Hafez YM, Attia K, Rashwan E, Husnain LA, AlGwaiz HI, Abdelaal KA (2021) Evaluation of silicon and proline application on the oxidative machinery in drought-stressed sugar beet. Antioxid 10:398

    Article  CAS  Google Scholar 

  • Arora A, Sairam RK, Srivastava GC (2002) Oxidative stress and antioxidative system in plants. Curr Sci 25:1227–1238

    Google Scholar 

  • Arrom L, Munné-Bosch S (2010) Tocopherol composition in flower organs of Lilium and its variations during natural and artificial senescence. Plant Sci 179:289–295

    Article  CAS  Google Scholar 

  • Aspinal D, Paleg G (1981) Proline accumulation: physiological aspects. In: Paleg LG, Aspinall D (eds) The Physiology and Biochemistry of Drought Resistance in Plants. Academic Press, Sydney, Australia, pp 280–295

    Google Scholar 

  • Ataii D, Naderi R, Khandan-Mirkohi A (2015) Exogenous putrescine delays senescence of Lisianthus cut flowers. J Ornam Plants 5:167–174

    Google Scholar 

  • Axerold B, Chesbrough TM, Laakso S (1981) Lipoxygenase from soybean. In: Lowenstein JM (ed) Meth Enzymol. Academic press, New York, pp 441–451

    Google Scholar 

  • Bartoli CG, Simontacchi M, Montaldi E, Puntarulo S (1996) Oxidative stress, antioxidant capacity and ethylene production during ageing of cut carnation (Dianthus caryophyllus) petals. J Exp Bot 47:595–601

    Article  CAS  Google Scholar 

  • Chakrabarty D, Chatterjee J, Datta SK (2007) Oxidative stress and antioxidant activity as the basis of senescence in chrysanthemum florets. Plant Growth Regul 53:107–115

    Article  CAS  Google Scholar 

  • Chakrabarty D, Verma AK, Datta SK (2009) Oxidative stress and antioxidant activity as the basis of senescence in Hemerocallis (day lily) flowers. J Hortic 1:113–119

    Google Scholar 

  • Chen GX, Asada K (1989) Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol 30:987–998

    Article  CAS  Google Scholar 

  • Dar RA, Tahir I, Ahmad SS (2015) Is the biochemical mechanism of petal senescence similar within a genus? A case study of Dianthus. Hortic Environ Biotechnol 56:654–661

    Article  CAS  Google Scholar 

  • Dar MI, Naikoo MI, Rehman F, Naushin F, Khan FA (2016) Proline accumulation in plants: roles in stress tolerance and plant development. In: Osmolytes and plants acclimation to changing environment: emerging omics technologies. Springer, New Delhi p 155-166

  • Dhindsa RS, Plumb-Dhindsa PA, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101

    Article  CAS  Google Scholar 

  • El-Shawa GM, Rashwan EM, Abdelaal KA (2020) Mitigating salt stress effects by exogenous application of proline and yeast extract on morpho-physiological, biochemical and anatomical characters of calendula plants. Sci J Flowers Ornam Plants 7:461–482

    Article  Google Scholar 

  • Forlani G, Trovato M, Funck D, Signorelli S (2019) Regulation of proline accumulation and its molecular and physiological functions in stress defence. In: Osmoprotectant-mediated abiotic stress tolerance in plants Springer, Cham p. 73–97

  • Fukuchi-Mizutani M, Ishiguro K, Nakayama T, Utsunomiya Y, Tanaka Y, Kusumi T, Ueda T (2000) Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence. Plant Sci 160:129–137

    Article  CAS  PubMed  Google Scholar 

  • Ghaffari H, Tadayon MR, Bahador M, Razmjoo J (2021) Investigation of the proline role in controlling traits related to sugar and root yield of sugar beet under water deficit conditions. Agric Water Manag 243:106448

  • Halliwell B (2006) Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physio 141:312–322

    Article  CAS  Google Scholar 

  • Hanif S, Saleem MF, Sarwar M, Irshad M, Shakoor A, Wahid MA, Khan HZ (2020) Biochemically triggered heat and drought stress tolerance in rice by proline application. J Plant Growth Regul. https://doi.org/10.1007/s00344-020-10095-3

    Article  Google Scholar 

  • Hasanuzzaman M, Alam M, Rahman A, Hasanuzzaman M, Nahar K, Fujita M (2014) Exogenous proline and glycine betaine mediated upregulation of antioxidant defense and glyoxalase systems provides better protection against salt-induced oxidative stress in two rice (Oryza sativa L.) varieties. Biomed Res Int 2014:17

    Google Scholar 

  • Hayat S, Hayat Q, Alyemeni MN, Wani AS, Pichtel J, Ahmad A (2012) Role of proline under changing environments: a review. Plant Signal Behav 7:1456–1466

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  CAS  PubMed  Google Scholar 

  • Hossain Z, Mandal AKA, Datta SK, Biswas AK (2006) Decline in ascorbate peroxidase activity–A prerequisite factor for tepal senescence in gladiolus. J Plant Physiol 163:186–194

    Article  CAS  PubMed  Google Scholar 

  • Kumar N, Pal M, Srivastava GC (2009) Proline metabolism in senescing rose petals (Rosa hybrida L. ‘First Red’). J Hortic Sci Biotechnol 84:536–540

    Article  CAS  Google Scholar 

  • Kumar N, Pal M, Singh A, SaiRam RK, Srivastava GC (2010) Exogenous proline alleviates oxidative stress and increase vase life in rose (Rosa hybrida L. ‘Grand Gala’). Sci Hortic 127:79–85

    Article  CAS  Google Scholar 

  • Kumar V, Khare T, Srivastav A, Surekha C, Shriram V, Wani SH (2019) Oxidative stress and leaf senescence: important insights. In: Senescence Signalling and Control in Plants Academic Press p. 139–163

  • Kwok D, Shetty K (1998) Effects of proline and proline analogs on total phenolic and rosmarinic acid levels in shoot clones of thyme (Thymus vulgaris L.). J Food Biochem 22:37–51

    Article  CAS  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    Article  CAS  PubMed  Google Scholar 

  • Mattioli R, Costantino P, Trovato M (2009) Proline accumulation in plants: not only stress. Plant Signal Behav 4:1016–1018

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mishra S, Dubey RS (2006) Inhibition of ribonuclease and protease activities in arsenic exposed rice seedlings: role of proline as enzyme protectant. J Plant Physiol 163:927–936

    Article  CAS  PubMed  Google Scholar 

  • Mohammadrezakhani S, Hajilou J, Rezanejad F, Zaare-Nahandi F (2019) Assessment of exogenous application of proline on antioxidant compounds in three Citrus species under low temperature stress. J Plant Interact 14:347–358

    Article  CAS  Google Scholar 

  • Moustakas M, Sperdouli I, Kouna T, Antonopoulou CI, Therios I (2011) Exogenous proline induces soluble sugar accumulation and alleviates drought stress effects on photosystem II functioning of Arabidopsis thaliana leaves. Plant Growth Regul 65:315–325

    Article  CAS  Google Scholar 

  • Nakhaie A, Habibi G, Vaziri A (2020) Exogenous proline enhances salt tolerance in acclimated Aloe vera by modulating photosystem II efficiency and antioxidant defense. S Afr J Bot. https://doi.org/10.1016/j.sajb.2020.06.005

    Article  Google Scholar 

  • Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153:375–380

    Article  CAS  Google Scholar 

  • Nisar S, Dar RA, Tahir I (2021) Salicylic acid retards senescence and makes flowers last longer in Nicotiana plumbaginifolia (Viv). Plant Physiol Rep 26:128–136

    Article  CAS  Google Scholar 

  • Ozden M, Demirel U, Kahraman A (2009) Effects of proline on antioxidant system in leaves of grapevine (Vitis vinifera L.) exposed to oxidative stress by H2O2. Sci Hortic 119:163–168

    Article  CAS  Google Scholar 

  • Peary JS, Prince TA (1990) Floral lipoxygenase: activity during senescence and inhibition by phenidone. J Am Soc Hortic 115:455–457

    Article  CAS  Google Scholar 

  • Rady Taha Mahdi nMRSAH (2016) Proline enhances growth, productivity and anatomy of two varieties of Lupinus termis L. grown under salt stress. S Afr J Bot 102:221–227

    Article  CAS  Google Scholar 

  • Rogers HJ (2012) Is there an important role for reactive oxygen species and redox regulation during floral senescence? Plant Cell Environ 35:217–233

    Article  CAS  PubMed  Google Scholar 

  • Rogers H, Munné-Bosch S (2016) Production and scavenging of reactive oxygen species and redox signaling during leaf and flower senescence: similar but different. Plant Physiol 171:1560–1568

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rouet-Mayer MA, Bureau JM, Lauriere C (1992) Identification and characterization of lipoxygenase isoforms in senescing carnation petals. Plant Physiol 98:971–978

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rubinstein B (2000) Regulation of cell death in flower petals. In: Lam E, Fukuda H, Greenberg J (eds) Programmed Cell Death in Higher Plants. Springer, Dordrecht, pp 59–74

    Chapter  Google Scholar 

  • Saeed T, Hassan I, Abbasi NA, Jilani G (2016) Antioxidative activities and qualitative changes in gladiolus cut flowers in response to salicylic acid application. Sci Hortic 210:236–241

    Article  CAS  Google Scholar 

  • Sairam RK (1994) Effect of moisture-stress on physiological activities of two contrasting wheat genotypes. Indian J Exp Biol 32:594–594

    Google Scholar 

  • Seleiman MF, Semida WM, Rady nM, Mohamed GF, Hemida KA, Alhammad BA, Shami A (2020) Sequential application of antioxidants rectifies ion imbalance and strengthens antioxidant systems in salt-stressed cucumber. Plants 9:1783

    Article  CAS  PubMed Central  Google Scholar 

  • Shinde S, Villamor JG, Lin W, Sharma S, Verslues PE (2016) Proline coordination with fatty acid synthesis and redox metabolism of chloroplast and mitochondria. Plant Physiol 172:1074–1088

    CAS  PubMed  PubMed Central  Google Scholar 

  • Swain T, Hillis WE (1959) The phenolic constituents of Prunus domestica. I.—The quantitative analysis of phenolic constituents. J Sci Food Agric 10:63–68

    Article  CAS  Google Scholar 

  • Szabados L, Savouré A (2010) Proline: a multifunctional amino acid. Trends Plant Sci 15:89–97

    Article  CAS  PubMed  Google Scholar 

  • Trippi V, Paulin A (1984) The senescence of cut carnations: a phasic phenomenon. Physiol Plant 60:221–226

    Article  CAS  Google Scholar 

  • Wang C, Lü P, Zhong S, Chen H, Zhou B (2017) LcMCII-1 is involved in the ROS-dependent senescence of the rudimentary leaves of Litchi chinensis. Plant Cell Rep 36:89–102

    Article  CAS  PubMed  Google Scholar 

  • Zhang Y, Guo WM, Chen SM, Han L, Li ZM (2007) The role of N-lauroylethanolamine in the regulation of senescence of cut carnations (Dianthus caryophyllus). J Plant Physiol 164:993–1001

    Article  CAS  PubMed  Google Scholar 

  • Zimmermann P, Zentgraf U (2005) The correlation between oxidative stress and leaf senescence during plant development. Cell Mol Biol Lett 10:515

    CAS  PubMed  Google Scholar 

  • Zouari M, Hassena AB, Trabelsi L, Rouina BB, Decou R, Labrousse P (2019) Exogenous proline-mediated abiotic stress tolerance in plants: Possible mechanisms. Osmoprotectant-Mediated Abiotic Stress Tolerance in Plants. Springer, Cham, pp 99–121

    Chapter  Google Scholar 

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Authors and Affiliations

  1. Plant Physiology and Biochemistry Research Laboratory, Department of Botany, University of Kashmir, Srinagar, 190006, India

    Shazia Parveen, Foziya Altaf, Sumira Farooq, Aehsan ul Haq, Mohammad Lateef Lone & Inayatullah Tahir

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  1. Shazia Parveen
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  2. Foziya Altaf
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Correspondence to Inayatullah Tahir.

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Parveen, S., Altaf, F., Farooq, S. et al. Is proline the quintessential sentinel of plants? A case study of postharvest flower senescence in Dianthus chinensis L.. Physiol Mol Biol Plants 27, 1597–1607 (2021). https://doi.org/10.1007/s12298-021-01028-9

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