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Genetic dissection of days to flowering via genome-wide association studies in Turkish common bean germplasm
Physiology and Molecular Biology of Plants ( IF 3.4 ) Pub Date : 2021-07-02 , DOI: 10.1007/s12298-021-01029-8
Muhammad Azhar Nadeem 1 , Ephrem Habyarimana 2 , Tolga Karaköy 1 , Faheem Shehzad Baloch 1
Affiliation  

Common bean is a nutrient‐dense legume crop serving as a source of food for millions of people. Characterization of unexplored common bean germplasm to unlock the phenotypic and genetic variations is still needed to explore the breeding potential of this crop. The current study aimed to dissect the genetic basis having association for days to flowering (DF). A total of 188 common bean accessions collected from 19 provinces of Turkey were used as plant material under five environments and two locations. Analysis of variance (ANOVA) revealed that genotypes and genotype by environment interaction have significant effects on DF. A total of 10 most stable accessions were evaluated from stability analysis. Overall maximum (75) and minimum (54) DF were observed for Hakkari-51 and Mus-46 accessions, respectively. The implemented constellation plot divided studied germplasm according to their DF and growth habit. A total of 7900 DArTseq markers were used for association analysis. Mixed linear model using the Q + K Model resulted a total of 18 DArTseq markers from five environments. DArT-8668385 marker identified in Bolu during 2016 was also associated with DF in Sivas during 2017. Combined data of five years resulted a total of four markers (DArT-22346534, DArT-3369768, DArT-3374613, and DArT-3370801) having significant association ( p  < 0.01 ) for DF. DArT-22346534 present on Pv 08 accounted a maximum of 9.89% variation to the studied trait. A total of four putative candidate genes were predicted from sequences reflecting homology to identified four DArTseq markers. We envisage that exploitation of identified DArTseq markers will hopefully beneficial for the development of new common bean varieties having better adaptation ability to changing climatic conditions.



中文翻译:

通过土耳其普通豆种质的全基因组关联研究对开花天数进行遗传解剖

普通豆是一种营养丰富的豆类作物,是数百万人的食物来源。仍然需要对未开发的普通豆种质进行表征以揭示表型和遗传变异,以探索这种作物的育种潜力。目前的研究旨在剖析与开花天数(DF)相关的遗传基础。从土耳其 19 个省收集的总共 188 份普通豆种质被用作在五个环境和两个地点下的植物材料。方差分析 (ANOVA) 表明基因型和环境相互作用的基因型对 DF 有显着影响。从稳定性分析中评估了总共 10 个最稳定的种质。对于 Hakkari-51 和 Mus-46 种质,分别观察到总体最大 (75) 和最小 (54) DF。实施的星座图根据其DF和生长习性划分研究的种质。共有 7900 个 DArTseq 标记用于关联分析。使用 Q + K 模型的混合线性模型产生了来自五个环境的总共 18 个 DArTseq 标记。2016 年在 Bolu 中发现的 DArT-8668385 标记也与 2017 年在 Sivas 中的 DF 相关。五年的综合数据得出共有四个标记(DArT-22346534、DArT-3369768、DArT-3374613 和 DArT-3370801)具有显着性协会 (p  < 0.01 ) 对于 DF。Pv 08 上存在的 DArT-22346534 对所研究性状的变异最大为 9.89%。从反映同源性的序列预测了总共四个推定的候选基因,以识别四个 DArTseq 标记。我们设想,利用已识别的 DArTseq 标记有望有助于开发具有更好适应气候条件变化能力的新普通豆品种。

更新日期:2021-07-02
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