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Genetic dissection of days to flowering via genome-wide association studies in Turkish common bean germplasm

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Abstract

Common bean is a nutrient‐dense legume crop serving as a source of food for millions of people. Characterization of unexplored common bean germplasm to unlock the phenotypic and genetic variations is still needed to explore the breeding potential of this crop. The current study aimed to dissect the genetic basis having association for days to flowering (DF). A total of 188 common bean accessions collected from 19 provinces of Turkey were used as plant material under five environments and two locations. Analysis of variance (ANOVA) revealed that genotypes and genotype by environment interaction have significant effects on DF. A total of 10 most stable accessions were evaluated from stability analysis. Overall maximum (75) and minimum (54) DF were observed for Hakkari-51 and Mus-46 accessions, respectively. The implemented constellation plot divided studied germplasm according to their DF and growth habit. A total of 7900 DArTseq markers were used for association analysis. Mixed linear model using the Q + K Model resulted a total of 18 DArTseq markers from five environments. DArT-8668385 marker identified in Bolu during 2016 was also associated with DF in Sivas during 2017. Combined data of five years resulted a total of four markers (DArT-22346534, DArT-3369768, DArT-3374613, and DArT-3370801) having significant association ( p  <  0.01 ) for DF. DArT-22346534 present on Pv 08 accounted a maximum of 9.89% variation to the studied trait. A total of four putative candidate genes were predicted from sequences reflecting homology to identified four DArTseq markers. We envisage that exploitation of identified DArTseq markers will hopefully beneficial for the development of new common bean varieties having better adaptation ability to changing climatic conditions.

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Acknowledgements

The authors express their gratitude to Scientific and Technological Research Council of Turkey (TÜBİTAK) to FSB for providing research grants for this study under the project no: TOVAG-2015O630.

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  1. Faculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, 58140, Sivas, Turkey

    Muhammad Azhar Nadeem, Tolga Karaköy & Faheem Shehzad Baloch

  2. CREA Research Center for Cereal and Industrial Crops, 40128, Bologna, Italy

    Ephrem Habyarimana

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  1. Muhammad Azhar Nadeem
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Nadeem, M.A., Habyarimana, E., Karaköy, T. et al. Genetic dissection of days to flowering via genome-wide association studies in Turkish common bean germplasm. Physiol Mol Biol Plants 27, 1609–1622 (2021). https://doi.org/10.1007/s12298-021-01029-8

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