In the process of glycosyltransferase-catalyzed transglycosylation, the synthesis of uridine diphosphate sugars is generally thermodynamically disfavored. This study found that GT_BP1 from Bacillus pumilus BF1 displayed the potential to reversely synthesize uridine diphosphate glucose (UDPG, UDP-glucose), which catalyzed the deglycosylation of ponasteroside A and the transglycosylation of phenolic compounds in one-pot reaction. Sequence alignment and phylogenetic tree analysis of GT_BP1 and other GTs with reversible glycosylation ability were also implemented. Using solvent engineering strategy, the reaction time in water-organic biphasic was shortened and the conversion was improved. The final conversion of ponasterone A was reached 93.4%, and the final yield of ferulic acid glycoside was 92.7%. Using the fed-batch technology with the coupled reaction, the cumulative product of ponasterone A was about 1.97 g/L, and the level of produced ferulic acid glycoside was about 1.52 g/L. The substrate specificity of the GT_BP1 was also confirmed. It implies the diversity of GT_BP1’s ability to construct UDP cycles.

在糖基转移酶催化的转糖基化过程中，尿苷二磷酸糖的合成在热力学上通常是不利的。本研究发现来自短小芽孢杆菌BF1的GT _BP1显示出逆向合成尿苷二磷酸葡萄糖（UDPG，UDP-葡萄糖）的潜力，其在一锅反应中催化松甾苷A的去糖基化和酚类化合物的转糖基化。GT _BP1的序列比对和系统树分析和其他具有可逆糖基化能力的 GTs 也被实施。采用溶剂工程策略，缩短了水-有机双相反应时间，提高了转化率。ponasterone A的最终转化率达到93.4%，阿魏酸糖苷的最终收率为92.7%。使用耦合反应的分批补料技术，松甾酮A的累积产物约为1.97 g/L，产生的阿魏酸糖苷水平约为1.52 g/L。GT _BP1的底物特异性也得到证实。这意味着 GT _BP1构建 UDP 循环的能力的多样性。