Astrocytes play an important role in the central nervous system function and may contribute to brain plasticity response during static magnetic fields (SMF) brain therapy. However, most studies evaluate SMF stimulation in brain plasticity while few studies evaluate the consequences of SMF at the cellular level. Thus, we here evaluate the effects of SMF at 305 mT (medium-intensity) in a primary culture of healthy/normal cortical astrocytes obtained from neonatal (1 to 2-day-old) Wistar rats. After reaching confluence, cells were daily subjected to SMF stimulation for 5 min, 15 min, 30 min, and 40 min during 7 consecutive days. Oxidative stress parameters, cell cycle, cell viability, and mitochondrial function were analyzed. The antioxidant capacity was reduced in groups stimulated for 5 and 40 min. Although no difference was observed in the enzymatic activity of superoxide dismutase and catalase or the total thiol content, lipid peroxidation was increased in all stimulated groups. The cell cycle was changed after 40 min of SMF stimulation while 15, 30, and 40 min led cells to death by necrosis. Mitochondrial function was reduced after SMF stimulation, although imaging analysis did not reveal substantial changes in the mitochondrial network. Results mainly revealed that SMF compromised healthy astrocytes’ oxidative status and viability. This finding reveals how important is to understand the SMF stimulation at the cellular level since this therapeutic approach has been largely used against neurological and psychiatric diseases.

星形胶质细胞在中枢神经系统功能中发挥着重要作用，可能有助于静磁场（SMF）脑治疗期间的大脑可塑性反应。然而，大多数研究评估 SMF 刺激对大脑可塑性的影响，而很少有研究评估 SMF 在细胞水平上的影响。因此，我们在这里评估了 305 mT（中等强度）的 SMF 对从新生（1 至 2 天龄）Wistar 大鼠获得的健康/正常皮质星形胶质细胞的原代培养物的影响。达到汇合后，连续 7 天每天对细胞进行 SMF 刺激 5 分钟、15 分钟、30 分钟和 40 分钟。分析了氧化应激参数、细胞周期、细胞活力和线粒体功能。刺激 5 分钟和 40 分钟的组的抗氧化能力有所降低。尽管超氧化物歧化酶和过氧化氢酶的酶活性或总硫醇含量没有观察到差异，但所有刺激组中脂质过氧化均增加。 SMF刺激40分钟后细胞周期发生变化，而15、30和40分钟则导致细胞坏死死亡。尽管成像分析没有揭示线粒体网络的实质性变化，但 SMF 刺激后线粒体功能降低。结果主要表明，SMF 损害了健康星形胶质细胞的氧化状态和活力。这一发现揭示了在细胞水平上理解 SMF 刺激的重要性，因为这种治疗方法已主要用于治疗神经和精神疾病。