N-acetylcysteine ameliorate cytotoxic injury in piglets sertoli cells induced by zearalenone and deoxynivalenol,Environmental Science and Pollution Research

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N-acetylcysteine ameliorate cytotoxic injury in piglets sertoli cells induced by zearalenone and deoxynivalenol
Environmental Science and Pollution Research Pub Date : 2021-06-22 , DOI: 10.1007/s11356-021-14052-9
Li Cao ₁ , Jie Zhao ₁ , Jingru Xu ₁ , Lei Zhu ₁ , Sajid Ur Rahman _{1,

2} , Shibin Feng ₁ , Yu Li _{1,

3} , Jinjie Wu _{1,

3} , Xichun Wang _{1,

3}

Affiliation

Zearalenone (ZEA) and Deoxynivalenol (DON) are two mycotoxins highly detected in agricultural products and feed. Both mycotoxins produce reproductive toxicity and pose a serious threat to human and animal health, among which pigs are the most sensitive animals. Sertoli cells (SCs) play an important role in spermatogenesis; however, the combined toxicity of ZEA and DON and the screening of effective protective agents remains to be determined. By studying the effects of N-acetylcysteine (NAC) on the cells exposed to 20 μM of ZEA and 0.6 μM of DON, we explored the protective mechanism of NAC (4 mM) on the cytotoxic injury of piglets SCs induced by both mycotoxins. The results showed that the combination of ZEA and DON destroy organelles and SCs structures, NAC significantly alleviates the damage caused by ZEA and DON. NAC also significantly increased the expression and distribution of zonula occludens 1 (ZO-1), decreased the relative mRNA and protein expression levels of Bax, Bid, caspase-3, and caspase-9, and increased Bcl-2 expression level and inhibited the decrease of mitochondrial membrane potential. Further, NAC also eases the cell cycle arrest and oxidative stress caused by ZEA and DON. In summary, our results show that NAC could alleviate SCs injury via reducing the oxidative damage and apoptosis caused by ZEA and DON.

Graphical abstract

中文翻译：

N-乙酰半胱氨酸改善玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇诱导的仔猪支持细胞的细胞毒性损伤

玉米赤霉烯酮 (ZEA) 和脱氧雪腐镰刀菌烯醇 (DON) 是在农产品和饲料中高度检出的两种真菌毒素。这两种霉菌毒素都会产生生殖毒性，严重威胁人类和动物的健康，其中猪是最敏感的动物。支持细胞 (SCs) 在精子发生中起重要作用；然而，ZEA 和 DON 的联合毒性以及有效保护剂的筛选仍有待确定。通过研究 N-乙酰半胱氨酸 (NAC) 对暴露于 20 μM ZEA 和 0.6 μM DON 的细胞的影响，我们探索了 NAC (4 mM) 对两种真菌毒素诱导的仔猪 SCs 细胞毒性损伤的保护机制。结果表明，ZEA 和 DON 的结合破坏了细胞器和 SCs 结构，NAC 显着减轻了 ZEA 和 DON 造成的损伤。NAC还显着增加了封闭带1（ZO-1）的表达和分布，降低了Bax、Bid、caspase-3和caspase-9的相对mRNA和蛋白表达水平，增加了Bcl-2的表达水平并抑制了线粒体膜电位降低。此外，NAC 还可以缓解由 ZEA 和 DON 引起的细胞周期停滞和氧化应激。总之，我们的结果表明 NAC 可以通过减少由 ZEA 和 DON 引起的氧化损伤和细胞凋亡来减轻 SCs 损伤。NAC 还可以缓解由 ZEA 和 DON 引起的细胞周期停滞和氧化应激。总之，我们的结果表明 NAC 可以通过减少由 ZEA 和 DON 引起的氧化损伤和细胞凋亡来减轻 SCs 损伤。NAC 还可以缓解由 ZEA 和 DON 引起的细胞周期停滞和氧化应激。总之，我们的结果表明 NAC 可以通过减少由 ZEA 和 DON 引起的氧化损伤和细胞凋亡来减轻 SCs 损伤。

图形概要

更新日期：2021-06-22

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