Planetary centrifugal bioreactors are promising candidates for cell culture platforms since there is no pollution caused by stirring blades. In this work, the fluid structure in a planetary centrifugal bioreactor was investigated using the computational fluid dynamics (CFD) method. The effects of operating conditions on the oxygen transfer rate (OTR), mixing efficiency and shear environment of the bioreactor were studied with the revolution speed (N) ranging from 60 to 160 rpm and the rotation-to-revolution speed ratio (i) from −2 to 1. The results show that the volumetric mass transfer coefficient (k_La), turbulence intensity, volumetric power consumption, and shear stress increase along with the increase of the revolution and rotation speeds. Furthermore, the rotation in the opposite direction to the revolution is beneficial to the performance of the bioreactor. The planetary centrifugal bioreactor has a higher k_La of 50–200/h and a lower average shear stress of 0.01–0.05 Pa in comparison with conventional stirred tank bioreactors, which makes it suitable for biological culture of oxygen-consuming cells and shear-sensitive cells.

行星离心生物反应器是细胞培养平台的有希望的候选者，因为没有搅拌叶片造成的污染。在这项工作中，使用计算流体动力学 (CFD) 方法研究了行星式离心生物反应器中的流体结构。研究了操作条件对生物反应器的氧气转移率 (OTR)、混合效率和剪切环境的影响，转速 (N) 范围为 60 至 160 rpm，自转转速比 (i) 为−2 比 1。 结果表明，体积传质系数 (k _La) 湍流强度、体积功率消耗和剪切应力随着转速和转速的增加而增加。此外，与公转方向相反的自转有利于生物反应器的性能。与传统的搅拌釜生物反应器相比，行星离心生物反应器的 k _L a 较高，为 50-200/h，平均剪切应力较低，为 0.01-0.05 Pa，适用于耗氧细胞的生物培养和剪切-敏感细胞。