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Analysis of Ecological Networks in Multicomponent Communities of Microorganisms: Possibilities, Limitations, and Potential Errors
Russian Journal of Ecology ( IF 1.0 ) Pub Date : 2021-06-11 , DOI: 10.1134/s1067413621030085
V. S. Mikryukov , O. V. Dulya , G. A Likhodeevskii , E. L. Vorobeichik

Abstract—

Owing to the high resolving power and efficiency of DNA sequencing, researchers have discovered the extremely high diversity of bacterial, fungal, protist, and microinvertebrate communities in the soil, wood, phyllosphere, and other natural media. Studies on the properties of these communities require powerful tools for analyzing multicomponent systems. One of them is the analysis of ecological networks, which makes it possible to solve a broad range of problems. This review briefly describes the possibilities of network analysis, its concepts, and metrics of network topology.It also indicates limitations related to specific features of DNA sequencing (compositionality and data sparsity) and potential sources of errors in the interpretation of results (relic DNA, artifactual DNA sequences and spurious connections in a network). The focus is on the communities of microorganisms, but the discussed issues are relevant for most other groups of the biota.



中文翻译:

微生物多组分群落生态网络分析:可能性、局限性和潜在错误

摘要-

由于 DNA 测序的高分辨能力和效率,研究人员发现了土壤、木材、叶际和其他自然介质中细菌、真菌、原生生物和微无脊椎动物群落的高度多样性。对这些群落特性的研究需要强大的工具来分析多组件系统。其中之一是对生态网络的分析,这使得解决广泛的问题成为可能。这篇综述简要描述了网络分析的可能性、它的概念和网络拓扑的度量。它还指出了与 DNA 测序的特定特征(组合性和数据稀疏性)和结果解释中的潜在错误来源(遗物 DNA、人为的 DNA 序列和网络中的虚假连接)。

更新日期:2021-06-13
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