Newly isolated Basic Red 9-degrading bacteria were isolated from textile wastewater using a pretreatment method. Nine strains were isolated; however, only five strains accumulated polyhydroxyalkanoates (PHAs). Thereafter, PHA-producing strains were identified through 16S rDNA sequencing analysis and phylogenetic evaluation and were found to belong to Enterobacter with 100% identification. The five isolated strains were incubated with a PHAs-producing medium containing 100 mg/l Basic Red 9 (BR9) to study decolorization efficiency, and PHAs production. Enterobacter sp. strains TS3 and TS1L effectively decolorized the BR9 dye with degradation rates of 63.43% and 79.15%, respectively. PHAs production from TS3 and TS1L was also observed to be 75.34% and 72.32% of dry cell weight (DCW), respectively. Furthermore, Enterobacter sp. strains TS3 and TS1L accumulated medium-co-long-chain-length PHAs (mcl-co-lcl PHAs). This is the first report using Enterobacter strains to degrade BR9 dyes from textile wastewater and to assess their ability to produce mcl-co-lcl PHAs.

使用预处理方法从纺织废水中分离出新分离的碱性红 9 降解细菌。分离出九株；然而，只有 5 个菌株积累了聚羟基链烷酸酯 (PHA)。此后，通过16S rDNA测序分析和系统发育评价鉴定出产PHA菌株，100%鉴定为肠杆菌属。将五个分离的菌株与含有 100 mg/l 碱性红 9 (BR9) 的产生 PHA 的培养基一起温育，以研究脱色效率和 PHA 的产生。肠杆菌sp. 菌株 TS3 和 TS1L 有效地使 BR9 染料脱色，降解率分别为 63.43% 和 79.15%。还观察到来自 TS3 和 TS1L 的 PHA 产量分别为干细胞重量 (DCW) 的 75.34% 和 72.32%。此外，肠杆菌属。TS3 和 TS1L 菌株积累了中等长度的 PHA（mcl-co-lcl PHA）。这是使用肠杆菌属菌株降解纺织废水中的 BR9 染料并评估其生产 mcl-co-lcl PHA 的能力的第一份报告。