Lactic acid bacteria (LAB) are among the most prevalent microorganisms forming the autochthonous microbiota of fruit. This study aimed to select LAB isolates with probiotic aptitudes from apple, banana, grape, and orange through evaluation of in vitro safety, technological, and functional-related properties. The ability of most promising selected isolates to survive in commercial apple and orange juice, meat stew, vegetable puree, and UHT milk during 28 days of refrigeration storage was evaluated. Ninety-three isolates identified preliminarily as LAB were recovered from fruit and 66 of these isolates passed safety tests. Most of these isolates were pre-identified as belonging to Lactobacillus or Enterococcus genus based on MALDI-ToF MS profiling. These 66 isolates were categorized into three homogeneous groups based on a preliminary cluster analysis run with data from experiments to measure technological characteristics. Nine LAB isolates were selected as the most promising for probiotic use based on a principal component analysis run with data from experiments to measure probiotic-related properties. Four of these isolates were sensitive to different antibiotics and identified (16S-rRNA gene sequencing) as Lactobacillus brevis (recently reclassified as L. brevis) or Lactobacillus spp. These 4 selected isolates had high viable counts and high percentages of physiologically active cells in apple and orange juice, beef stew, vegetable puree, and UHT milk during refrigeration storage. The results showed that apple, banana, orange, and grape are potential sources of LAB with aptitudes to be exploited for a possible probiotic use and distinguished abilities to survive in different food matrices.

乳酸菌 (LAB) 是形成水果本土微生物群的最普遍的微生物之一。本研究旨在通过评估体外安全性、技术和功能相关特性，从苹果、香蕉、葡萄和橙子中选择具有益生菌能力的 LAB 分离株。评估了最有希望的选定分离物在 28 天冷藏期间在商业苹果汁和橙汁、炖肉、蔬菜泥和 UHT 牛奶中存活的能力。从水果中回收了 93 株初步鉴定为 LAB 的菌株，其中 66 株通过了安全性测试。这些分离株中的大多数被预先确定为属于乳杆菌属或肠球菌属基于 MALDI-ToF MS 分析的属。根据使用来自实验的数据进行初步聚类分析，将这 66 个分离物分为三个同质组，以测量技术特性。根据使用实验数据运行的主成分分析，九种 LAB 分离株被选为最有希望用于益生菌的分离物，以测量益生菌相关特性。其中四种分离物对不同的抗生素敏感，并被鉴定（16S-rRNA 基因测序）为短乳杆菌（最近重新分类为短乳杆菌）或乳杆菌spp. 这 4 种选定的分离株在冷藏期间在苹果汁和橙汁、炖牛肉、蔬菜泥和 UHT 牛奶中具有高存活率和高百分比的生理活性细胞。结果表明，苹果、香蕉、橙子和葡萄是乳酸菌的潜在来源，具有被开发用于可能的益生菌用途和在不同食物基质中生存的显着能力。