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Selection of lactic acid bacteria with promising probiotic aptitudes from fruit and ability to survive in different food matrices

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Abstract

Lactic acid bacteria (LAB) are among the most prevalent microorganisms forming the autochthonous microbiota of fruit. This study aimed to select LAB isolates with probiotic aptitudes from apple, banana, grape, and orange through evaluation of in vitro safety, technological, and functional-related properties. The ability of most promising selected isolates to survive in commercial apple and orange juice, meat stew, vegetable puree, and UHT milk during 28 days of refrigeration storage was evaluated. Ninety-three isolates identified preliminarily as LAB were recovered from fruit and 66 of these isolates passed safety tests. Most of these isolates were pre-identified as belonging to Lactobacillus or Enterococcus genus based on MALDI-ToF MS profiling. These 66 isolates were categorized into three homogeneous groups based on a preliminary cluster analysis run with data from experiments to measure technological characteristics. Nine LAB isolates were selected as the most promising for probiotic use based on a principal component analysis run with data from experiments to measure probiotic-related properties. Four of these isolates were sensitive to different antibiotics and identified (16S-rRNA gene sequencing) as Lactobacillus brevis (recently reclassified as L. brevis) or Lactobacillus spp. These 4 selected isolates had high viable counts and high percentages of physiologically active cells in apple and orange juice, beef stew, vegetable puree, and UHT milk during refrigeration storage. The results showed that apple, banana, orange, and grape are potential sources of LAB with aptitudes to be exploited for a possible probiotic use and distinguished abilities to survive in different food matrices.

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Acknowledgements

Authors thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil) for partial funding of this research (Finance code 001) and Center for Strategic Technologies for Northeast (CETENE, Brazil) for support in identification of LAB isolates with MALDI-ToF MS.

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Authors and Affiliations

  1. Laboratório de Microbiologia de Alimentos, Departamento de Nutrição, Centro de Ciências da Saúde, Universidade Federal da Paraíba, Campus I, João Pessoa, Paraíba, Brazil

    Noádia Priscilla Araújo Rodrigues & Evandro Leite de Souza

  2. Departamento de Gastronomia, Centro de Tecnologia E Desenvolvimento Regional, Universidade Federal da Paraíba, Paraíba, João Pessoa, Brazil

    Noádia Priscilla Araújo Rodrigues & Estefânia Fernandes Garcia

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  1. Noádia Priscilla Araújo Rodrigues
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Contributions

Conceptualization: N.A. Rodrigues, E.F. Garcia, E.L. de Souza. Data curation; formal analysis: N.A. Rodrigues, E.F. Garcia, E.L. de Souza. Funding acquisition: E.L. de Souza. Methodology: N.A. Rodrigues, E.F. Garcia, E.L. de Souza. Project administration: E.L. de Souza. Supervision: E.F. Garcia, E.L. de Souza. Validation: N.A. Rodrigues, E.F. Garcia. Writing—original draft: N.A. Rodrigues, E.F. Garcia, E.L. de Souza. Writing—review and editing: E.L. de Souza.

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Correspondence to Evandro Leite de Souza.

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Rodrigues, N.P.A., Garcia, E.F. & de Souza, E.L. Selection of lactic acid bacteria with promising probiotic aptitudes from fruit and ability to survive in different food matrices. Braz J Microbiol 52, 2257–2269 (2021). https://doi.org/10.1007/s42770-021-00543-x

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