Purpose

Cystic echinococcosis (CE) is a zoonotic disease, caused by parasite known as Echinococcus granulosus sensu lato complex, leading to substantial economic losses in rural areas with public health problems. This study was carried out to understand the haplotypic profiles of the partial mitochondrial cytochrome c oxidase (mt-CO1) gene of cattle lung hydatid cyst samples which were obtained from two provinces in Turkey.

Methods

In this study, forty (n = 40) hydatid cyst samples from the lungs of slaughtered cattle were obtained. The germinal layers were taken separately for each individual cyst then stored in 70% ethanol. From each individual cyst sample, total genomic DNA was extracted. Amplification of the partial mt-CO1 gene (875 bp) was performed using a specific primer set by PCR, and then, the amplicons were sequenced. All sequences were analyzed individually, followed by alignment, and haplotype and phylogenetic analyses were then performed.

Results

By the sequence alignment process, 39 out of the 40 sequences were characterized as E. granulosus sensu stricto. However, one of them was matched with E. canadensis (G6/G7). The haplotype analyses of the E. granulosus s.s. isolates were arranged in a star-like orientation with a main haplotype, which was separated from other haplotypes by 1–10 mutation steps, and 26 haplotypes were identified. In the mt-CO1 sequences, 29 polymorphic sites were determined, and 34.5% (10/29) of them were parsimony informative.

Conclusions

The findings of this study provide the first report of E. canadensis (G6/G7 genotype) among cattle in Turkey.

中文翻译：

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牛肺包虫囊肿分离株的分子特征和单倍型分析以及土耳其牛分离株中加拿大棘球绦虫（G6/G7）的首次报告

目的

囊性棘球蚴病 (CE) 是一种人畜共患病，由被称为细粒棘球绦虫复合体的寄生虫引起，在存在公共卫生问题的农村地区造成重大经济损失。进行这项研究是为了了解从土耳其两个省获得的牛肺包虫包囊样品的部分线粒体细胞色素 c 氧化酶 (mt-CO1) 基因的单倍型谱。

方法

在这项研究中， 获得了来自屠宰牛肺的四十个 ( n = 40) 包虫囊肿样本。每个单独的孢囊分别取胚层，然后储存在 70% 的乙醇中。从每个单独的包囊样品中提取总基因组 DNA。部分mt-CO1基因(875 bp)的扩增使用特异性引物组通过PCR进行，然后对扩增子进行测序。对所有序列进行单独分析，然后进行比对，然后进行单倍型和系统发育分析。

结果

通过序列比对过程，40 个序列中的 39 个被表征为E. granulosus sensu stricto 。然而，其中之一与E. canadensis (G6/G7)匹配。E. granulosus ss 分离株的单倍型分析呈星状排列，其中一个主要单倍型通过1-10 个突变步骤与其他单倍型分开，共鉴定出26 个单倍型。在 mt-CO1 序列中，确定了 29 个多态位点，其中 34.5% (10/29) 是简约信息。

结论

这项研究的结果提供了土耳其牛群中加拿大 E. canadensis（G6/G7 基因型）的第一份报告。