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Molecular Characterization and Haplotype Analyses of Lung Hydatid Cyst Isolates of Cattle and First Report of Echinococcus canadensis (G6/G7) in Cattle Isolates in Turkey

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Abstract

Purpose

Cystic echinococcosis (CE) is a zoonotic disease, caused by parasite known as Echinococcus granulosus sensu lato complex, leading to substantial economic losses in rural areas with public health problems. This study was carried out to understand the haplotypic profiles of the partial mitochondrial cytochrome c oxidase (mt-CO1) gene of cattle lung hydatid cyst samples which were obtained from two provinces in Turkey.

Methods

In this study, forty (n = 40) hydatid cyst samples from the lungs of slaughtered cattle were obtained. The germinal layers were taken separately for each individual cyst then stored in 70% ethanol. From each individual cyst sample, total genomic DNA was extracted. Amplification of the partial mt-CO1 gene (875 bp) was performed using a specific primer set by PCR, and then, the amplicons were sequenced. All sequences were analyzed individually, followed by alignment, and haplotype and phylogenetic analyses were then performed.

Results

By the sequence alignment process, 39 out of the 40 sequences were characterized as E. granulosus sensu stricto. However, one of them was matched with E. canadensis (G6/G7). The haplotype analyses of the E. granulosus s.s. isolates were arranged in a star-like orientation with a main haplotype, which was separated from other haplotypes by 1–10 mutation steps, and 26 haplotypes were identified. In the mt-CO1 sequences, 29 polymorphic sites were determined, and 34.5% (10/29) of them were parsimony informative.

Conclusions

The findings of this study provide the first report of E. canadensis (G6/G7 genotype) among cattle in Turkey.

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Funding

This study has been supported by a grant from the Bingöl University Scientific Research Projects Unit (Project No: BAP-VF.2019.00.002).

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Veterinary Medicine, Bingol University, Bingol, Turkey

    Harun Kaya Kesik, Seyma Gunyakti Kilinc & Abdurrahman Gul

  2. Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119, Elazig, Turkey

    Figen Celik & Sami Simsek

  3. Department of Biosciences, COMSATS University Islamabad (CUI), Islamabad, Pakistan

    Haroon Ahmed

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  1. Harun Kaya Kesik
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  2. Figen Celik
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  4. Seyma Gunyakti Kilinc
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Contributions

HKK, FC, SGK and SS contributed to the conception and design of the study. HKK, FC, SGK and SS collected the samples, performed the laboratory activities and organized the database. HKK and SS wrote the manuscript. SS, HA and AG critically oversaw the substantial revisions of the manuscript.

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Correspondence to Sami Simsek.

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Kesik, H.K., Celik, F., Simsek, S. et al. Molecular Characterization and Haplotype Analyses of Lung Hydatid Cyst Isolates of Cattle and First Report of Echinococcus canadensis (G6/G7) in Cattle Isolates in Turkey. Acta Parasit. 66, 1538–1547 (2021). https://doi.org/10.1007/s11686-021-00432-1

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