Bio-particles are usually considered as an elastic material, while it has been proven that these particles such as viruses and cells behave more like viscoelastic materials because of the liquid cytoplasm in cells and also the protein capsid around viruses; thus, this property is not ignorable because it leads to a non-precise prediction in simulations. On the other hand, the surface of these particles is usually considered smooth, while the topography shows asperities on them. In this paper, the viscoelastic contact model along with the asperities’ distribution of the bio-particles is considered near reality. Simulation results reveal that the prediction of the viscoelastic contact models is more precise and closer to the experimental data than the elastic state. Using experimental results and applying their effect, asperities’ radii are obtained and comparison shows that the rough viscoelastic theory is more accurate and closer to the experimental data in comparison with viscoelastic models without asperity. The simulation of the first phase of the manipulation, applying three states of the elastic, the viscoelastic, and the rough viscoelastic in addition to a comparison with similar modes, shows that in the particle’s sliding on the substrate, tip sliding on the particle, and the particle rolling modes, the critical force has the highest magnitudes in the rough viscoelastic, the elastic, and the viscoelastic, respectively. However, results for the critical time are different, i.e., the highest critical times are related to the elastic, the viscoelastic, and the rough viscoelastic states, respectively.

生物颗粒通常被认为是一种弹性材料，但已经证明，由于细胞中的液体细胞质以及病毒周围的蛋白质衣壳，这些颗粒如病毒和细胞的行为更像是粘弹性材料；因此，此属性不可忽略，因为它会导致模拟中的预测不准确。另一方面，这些颗粒的表面通常被认为是光滑的，而地形则显示出它们的凹凸不平。在本文中，粘弹性接触模型以及生物颗粒的凹凸分布被认为是接近现实的。仿真结果表明，粘弹性接触模型的预测比弹性状态更精确，更接近实验数据。使用实验结果并应用它们的效果，获得了粗糙的半径，比较表明，与没有粗糙的粘弹性模型相比，粗粘弹性理论更准确，更接近实验数据。操纵的第一阶段的模拟，除了与相似模式的比较之外，还应用弹性、粘弹性和粗糙粘弹性三种状态，表明在粒子在基底上的滑动、尖端在粒子上的滑动以及在粒子滚动模式中，临界力分别在粗粘弹性、弹性和粘弹性中具有最高的量级。然而，临界时间的结果是不同的，即最高临界时间分别与弹性、粘弹性和粗粘弹性状态有关。