Aptamers, single-stranded DNAs/RNAs with a strong and specific interaction towards a target molecule, have wide applications in the fields of medicine and biosensors. In conventional aptamer selection methods, it is difficult to obtain “preorganized” and/or “induced-fit” type of aptamers selectively. In this study, separation and fractionation of single-stranded DNAs with/without stable preorganized structures were carried out using capillary sieving electrophoresis. The fractionated DNAs showed different mobilities and thermodynamic stabilities of their secondary structures; this outcome is deemed to be necessary for the synthesis of novel aptasensors with a desirable sensing mechanism.

适体是一种对靶分子具有强特异性相互作用的单链 DNA/RNA，在医学和生物传感器领域有着广泛的应用。在传统的适配体选择方法中，很难有选择地获得“预组织”和/或“诱导拟合”类型的适配体。在这项研究中，使用毛细管筛分电泳对具有/不具有稳定预组织结构的单链 DNA 进行分离和分级分离。分离后的DNA显示出不同的二级结构迁移率和热力学稳定性；这一结果被认为是合成具有理想传感机制的新型适体传感器所必需的。