Fractionation of Single-stranded DNAs with/without Stable Preorganized Structures Using Capillary Sieving Electrophoresis for Aptamer Selection

Masahide WADA; Tatsuro ENDO; Hideaki HISAMOTO; Kenji SUEYOSHI

doi:10.2116/analsci.21C003

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Fractionation of Single-stranded DNAs with/without Stable Preorganized Structures Using Capillary Sieving Electrophoresis for Aptamer Selection

Masahide WADA, Tatsuro ENDO, Hideaki HISAMOTO, Kenji SUEYOSHI

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Keywords: Aptamer selection, capillary sieving electrophoresis, structure-induced aptamer, structure-preorganized aptamer

JOURNAL FREE ACCESS

2021 Volume 37 Issue 6 Pages 799-802

DOI https://doi.org/10.2116/analsci.21C003

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Abstract

Aptamers, single-stranded DNAs/RNAs with a strong and specific interaction towards a target molecule, have wide applications in the fields of medicine and biosensors. In conventional aptamer selection methods, it is difficult to obtain “preorganized” and/or “induced-fit” type of aptamers selectively. In this study, separation and fractionation of single-stranded DNAs with/without stable preorganized structures were carried out using capillary sieving electrophoresis. The fractionated DNAs showed different mobilities and thermodynamic stabilities of their secondary structures; this outcome is deemed to be necessary for the synthesis of novel aptasensors with a desirable sensing mechanism.

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