Inflammatory bowel disease is a chronic, relapsing–remitting gastrointestinal disorder which has become a serious global concern, and it imposes a great degree of health and economic burdens on communities worldwide. Although the presence of non-Helicobacter pylori Helicobacter (NHPH) microorganisms has been reported in various gastrointestinal disorders, their putative role in the pathogenesis of IBD has been a matter of controversy. The present study aimed to investigate the existence of gastric and enterohepatic NHPHs and their probable coinfection with H. pylori in IBD. Totally, 168 clinical specimens including 70 colonic biopsies and 98 fecal specimens were obtained from IBD patients. Genomic DNA was extracted from all samples, and its quality and concentration were assessed by β-globin PCR and spectrophotometry. The Helicobacter genus-specific PCR was performed using 16S rRNA gene. All samples were also tested for H. pylori infection by PCR of ureC gene fragment (glmM). The presence of NHPH was examined by using species-specific PCR assays. Based on PCR results, H. pylori was detected in 12.9% and 3.1% of colonic biopsies and fecal specimens, respectively. However, no statistically significant correlation was observed (P value > 0.05). We failed to find NHPH in both colonic biopsies and fecal specimens from IBD patients. Despite the fact that none of the IBD patients harbored the NHPH in the current work, further cohorts with larger sample size are required to determine the possible relationship between NHPH infection and IBD pathogenesis.

炎症性肠病是一种慢性、复发缓解型胃肠道疾病，已成为全球严重关注的问题，给全世界社区带来很大的健康和经济负担。尽管已报道各种胃肠道疾病中存在非幽门螺杆菌(NHPH) 微生物，但它们在 IBD 发病机制中的假定作用一直存在争议。本研究旨在探讨 IBD 中胃和肠肝 NHPH 的存在及其与幽门螺杆菌的可能共感染。总共从 IBD 患者中获取了 168 份临床标本，其中包括 70 份结肠活检标本和 98 份粪便标本。从所有样品中提取基因组DNA，并通过β-珠蛋白PCR和分光光度法评估其质量和浓度。使用 16S rRNA 基因进行螺杆菌属特异性 PCR。所有样本还通过ureC基因片段 ( glmM ) 的 PCR 检测幽门螺杆菌感染。通过使用物种特异性 PCR 检测来检查 NHPH 的存在。根据PCR结果，在结肠活检和粪便标本中分别检测出12.9%和3.1%的幽门螺杆菌。然而，没有观察到统计学上显着的相关性（ P值> 0.05）。我们未能在 IBD 患者的结肠活检和粪便标本中发现 NHPH。尽管目前的工作中没有 IBD 患者携带 NHPH，但仍需要更大样本量的队列来确定 NHPH 感染与 IBD 发病机制之间可能的关系。