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A report of nonexistence of the non-Helicobacter pylori Helicobacter species in Iranian patients suffering from inflammatory bowel disease

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Abstract

Inflammatory bowel disease is a chronic, relapsing–remitting gastrointestinal disorder which has become a serious global concern, and it imposes a great degree of health and economic burdens on communities worldwide. Although the presence of non-Helicobacter pylori Helicobacter (NHPH) microorganisms has been reported in various gastrointestinal disorders, their putative role in the pathogenesis of IBD has been a matter of controversy. The present study aimed to investigate the existence of gastric and enterohepatic NHPHs and their probable coinfection with H. pylori in IBD. Totally, 168 clinical specimens including 70 colonic biopsies and 98 fecal specimens were obtained from IBD patients. Genomic DNA was extracted from all samples, and its quality and concentration were assessed by β-globin PCR and spectrophotometry. The Helicobacter genus-specific PCR was performed using 16S rRNA gene. All samples were also tested for H. pylori infection by PCR of ureC gene fragment (glmM). The presence of NHPH was examined by using species-specific PCR assays. Based on PCR results, H. pylori was detected in 12.9% and 3.1% of colonic biopsies and fecal specimens, respectively. However, no statistically significant correlation was observed (P value > 0.05). We failed to find NHPH in both colonic biopsies and fecal specimens from IBD patients. Despite the fact that none of the IBD patients harbored the NHPH in the current work, further cohorts with larger sample size are required to determine the possible relationship between NHPH infection and IBD pathogenesis.

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Acknowledgements

The authors would like to thank the sample collection team at Taleghani hospital and all the staffs in Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Funding

This study was supported financially by a grant [no. RIGLD 995] from Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

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Authors and Affiliations

  1. Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Samira Pirmanesh, Nasrin Mirzaei, Masoumeh Azimirad & Abbas Yadegar

  2. Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI, USA

    John Y. Kao

  3. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Hamid Asadzadeh Aghdaei

  4. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mohammad Reza Zali

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  1. Samira Pirmanesh
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  4. Abbas Yadegar
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Contributions

SP and MA performed the microbiological and molecular tests. AY and NM reviewed the literature and prepared the manuscript draft. AY designed the study, analyzed the data, and revised the manuscript. JYK, HAA, and MRZ provided clinical advice and critically revised the manuscript. All authors approved the final version of the manuscript and the authorship list.

Corresponding author

Correspondence to Abbas Yadegar.

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Ethical approval

The study was approved by the Institutional Ethical Review Committee of RIGLD at Shahid Beheshti University of Medical Sciences (Project No. IR.SBMU.RIGLD.REC.1396.188).

Informed consent

All the patients were asked to sign a written informed consent prior to their participation in the study.

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The authors declare no competing interests.

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Pirmanesh, S., Mirzaei, N., Azimirad, M. et al. A report of nonexistence of the non-Helicobacter pylori Helicobacter species in Iranian patients suffering from inflammatory bowel disease. Folia Microbiol 66, 751–759 (2021). https://doi.org/10.1007/s12223-021-00883-z

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