Enantiomerically pure (S)-1-(4-methoxyphenyl) ethanol is a significant molecule for the production of various drug intermediates. (S)-1-(4-methoxyphenyl) ethanol was synthesized from 4-methoxyacetophenone using Lactobacillus senmaizuke as a biocatalyst. In addition, optimization of experimental conditions is important to analyze the role of culture parameters for catalytic bioreduction reactions. For this particular purpose, the experimental conditions of pH, incubation period, temperature, and agitation speed were investigated with the Box–Behnken experimental design-based proposed optimization model. (S)-1-(4-methoxyphenyl) ethanol, which can be used for the synthesis of antihistamines, including diphenhydramine hydrochloride and loratadine cycloalkyl [b] indoles that have the treatment function for an allergic response, was obtained in > 99% conversion, > 99% enantiomeric excess and 96% yield with whole cells of L. senmaizukei at this optimization conditions: pH = 5.80, the temperature = 29 °C, incubation period = 50 h, and agitation speed = 155 rpm. The bioreduction of 4-methoxyacetophenone efficiency was importantly affected by the quadratic and linear effects of experimental design parameters. Besides, the results demonstrate the importance of design parameters for catalytic bioreduction reactions. It is also concluded that the results show the effectiveness of the Box–Behnken experimental design-based proposed model to obtain optimum operating conditions of design parameters for catalytic bioreduction reactions.

对映体纯 ( S )-1-(4-甲氧基苯基) 乙醇是生产各种药物中间体的重要分子。( S )-1-(4-甲氧基苯基) 乙醇是由 4-甲氧基苯乙酮合成的，使用仙麦祖乳杆菌作为生物催化剂。此外，实验条件的优化对于分析培养参数对催化生物还原反应的作用很重要。为此特定目的，使用基于 Box-Behnken 实验设计的拟议优化模型研究了 pH 值、孵育时间、温度和搅拌速度等实验条件。(秒)-1-(4-甲氧基苯基)乙醇，可用于合成抗组胺药，包括具有治疗过敏反应功能的苯海拉明盐酸盐和氯雷他定环烷基[b]吲哚，转化率>99%，> 99% 的对映体过量和 96% 的产率与L. senmaizukei全细胞在此优化条件下：pH = 5.80，温度 = 29 °C，孵育时间 = 50 小时，搅拌速度 = 155 rpm。4-甲氧基苯乙酮效率的生物还原受到实验设计参数的二次和线性效应的重要影响。此外，结果证明了催化生物还原反应设计参数的重要性。还得出结论，结果表明基于 Box-Behnken 实验设计的拟议模型在获得催化生物还原反应设计参数的最佳操作条件方面的有效性。