Microalgae are considered promising feedstocks for biofuel and bio-product generation. The algal carbohydrates can be hydrolyzed into sugars before their fermentation into ethanol. In this study, nutrient limitation strategy was employed to evaluate the biochemical composition of Chlorella sorokiniana. Limiting nitrate (1.0 g/L KNO₃) in the culture medium increased the total carbohydrate and starch content of microalga by 50.28 and 34.06%, respectively. However, this significantly lowered their yield due to low microalgal biomass production. Cultivation of C. sorokiniana cells with 4.0 g/L KNO₃ as nitrogen source for 8 days was optimum for bioethanol production as the highest total carbohydrate yield of 422.44 mg/L was obtained under these conditions. Nitrate limitation (1.0 g/L KNO₃) favored the increased production of high-value carotenoids in C. sorokiniana that could further contribute to improving the economics of the bioethanol production process. Feasibility studies for ethanol production from C. sorokiniana revealed that a maximum of 13.86 mg/mL of reducing sugars was extracted in the hydrolysate by treating the microalgal biomass with 2.8% sulfuric acid at 121 °C for 30 min. Fermentation of acid hydrolysate produced ethanol at a concentration of 2.91 mg/mL in 96 h with 41.16% of theoretical yield.

微藻被认为是生产生物燃料和生物产品的有前途的原料。藻类碳水化合物在发酵成乙醇之前可以水解成糖。在本研究中，采用营养限制策略来评估Chlorella sorokiniana的生化成分。限制培养基中的硝酸盐 (1.0 g/L KNO ₃ ) 使微藻的总碳水化合物和淀粉含量分别增加了 50.28% 和 34.06%。然而，由于微藻生物质产量低，这显着降低了它们的产量。用 4.0 g/L KNO ₃培养C. sorokiniana细胞作为氮源 8 天是生物乙醇生产的最佳选择，因为在这些条件下获得了 422.44 mg/L 的最高总碳水化合物产量。硝酸盐限制 (1.0 g/L KNO ₃ ) 有利于提高C. sorokiniana中高价值类胡萝卜素的产量，这可能进一步有助于提高生物乙醇生产过程的经济性。从C. sorokiniana生产乙醇的可行性研究表明，通过在 121°C 下用 2.8% 硫酸处理微藻生物质 30 分钟，在水解产物中提取了最多 13.86 mg/mL 的还原糖。酸水解产物的发酵在 96 小时内产生浓度为 2.91 mg/mL 的乙醇，理论产率为 41.16%。