Bluetongue virus (BTV) is an RNA virus that infects cattle and sheep. The objective of this study was to compare two real-time PCRs for the detection of BTV and to monitor Orbivirus viremia in sheep and cattle for 6 months. The PCR results showed the occurrence of infected animals throughout the experiment without records of clinical signs. The number of positive animals reduced during the experiment, but some animals were positive for BTV RNA during the entire experiment. The performance of the two RT-qPCRs for BTV detection techniques used in this work revealed a kappa index of 0.71 for cattle and 0.75 for sheep.

中文翻译：

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牛羊蓝舌病毒分子诊断及病毒血症研究

蓝舌病毒(BTV) 是一种感染牛和羊的 RNA 病毒。本研究的目的是比较两种实时 PCR 以检测 BTV 并监测绵羊和牛的环状病毒病毒血症 6 个月。PCR结果显示在整个实验过程中发生感染动物，没有临床症状记录。实验过程中阳性动物的数量减少了，但在整个实验过程中，一些动物的 BTV RNA 呈阳性。本工作中使用的两种用于 BTV 检测技术的 RT-qPCR 的性能显示，牛的 kappa 指数为 0.71，羊的 kappa 指数为 0.75。