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Study of molecular diagnosis and viremia of bluetongue virus in sheep and cattle

  • Veterinary Microbiology - Short Communication
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Abstract

Bluetongue virus (BTV) is an RNA virus that infects cattle and sheep. The objective of this study was to compare two real-time PCRs for the detection of BTV and to monitor Orbivirus viremia in sheep and cattle for 6 months. The PCR results showed the occurrence of infected animals throughout the experiment without records of clinical signs. The number of positive animals reduced during the experiment, but some animals were positive for BTV RNA during the entire experiment. The performance of the two RT-qPCRs for BTV detection techniques used in this work revealed a kappa index of 0.71 for cattle and 0.75 for sheep.

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Funding

This work was funded by Ministério da Agricultura, Pecuária e Abastecimento, INCT Pecuária, and CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnológico) SAGRES Project: 457417/2012–9.

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Authors and Affiliations

  1. Laboratório Nacional Agropecuário de Minas Gerais, Avenida Rômulo Joviano, Centro, Pedro Leopoldo, Minas Gerais, ZIP 33600-000, Brazil

    Marcela Gasparini, Mateus Laguardia-Nascimento, Érica Bravo Sales, Anna Gabriella Guimarães Oliveira, Marcelo Fernandes Camargos & Antônio Augusto Fonseca Júnior

  2. Department of Preventive Veterinary Medicine, Veterinary School, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil

    Zélia I. P. Lobato

  3. UNIFEMM – Centro Universitário de Sete Lagoas, Sete Lagoas, Minas Gerais, Brasil

    Antônio Augusto Fonseca Júnior

Authors
  1. Marcela Gasparini
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  2. Mateus Laguardia-Nascimento
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  3. Érica Bravo Sales
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  4. Anna Gabriella Guimarães Oliveira
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  5. Zélia I. P. Lobato
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  6. Marcelo Fernandes Camargos
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  7. Antônio Augusto Fonseca Júnior
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Contributions

Acquisition, analysis, or interpretation of data: Marcela Gasparini, Mateus Laguardia-Nascimento, Érica Bravo Sales, Anna Gabriella Guimarães Oliveira, Zélia IP Lobato, Antônio Augusto Fonseca Júnior. Drafted the work or revised it critically for important intellectual content: Antônio Augusto Fonseca Júnior.

Corresponding author

Correspondence to Antônio Augusto Fonseca Júnior.

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No animals were harmed during this study. Samples were collected as a routine for diagnostic of viral diseases in the official laboratory of the Brazilian Ministry of Agriculture, Livestock, and Supply.

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The authors declare no competing interests.

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Responsible Editor: Fernando R. Spilki

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Gasparini, M., Laguardia-Nascimento, M., Sales, É.B. et al. Study of molecular diagnosis and viremia of bluetongue virus in sheep and cattle. Braz J Microbiol 52, 1623–1626 (2021). https://doi.org/10.1007/s42770-021-00518-y

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  • DOI: https://doi.org/10.1007/s42770-021-00518-y

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