Numerous studies have demonstrated that lncRNAs participate in regulatory networks of different cancers. Dysregulation of various lncRNAs such as DUXAP8, LINC00963, and FOXD2-AS1 has been reported in the development of various cancers. The aim of this study was investigation of the importance and potential roles of DUXAP8, LINC00963, and FOXD2-AS1 in ER⁺ breast cancer (BC). We examined the expression levels of DUXAP8, LINC00963, and FOXD2-AS1 in 71 luminal A and B tumor tissues and two luminal A cell lines (MCF7 and T47D) compared with adjacent non-tumor tissues and MCF10A cell line by qRT-PCR assay, respectively. For identifying the relation between three lncRNAs and luminal BC, bioinformatic analyses were performed using some databases and software including GENEVESTIGATOR software, GEPIA2, DAVID, REVIGO, STRING, lncATLAS, Kaplan–Meier plotter, starBase, and miRNet tool. The results showed the significant upregulation of all three lncRNAs in luminal A and B tumor specimens and cell lines. Upregulation of DUXAP8 and FOXD2-AS1 was significantly associated with progesterone receptor-positive (PR⁺) and p53 protein expression in luminal BC patients, respectively. Based on bioinformatic analyses, DUXAP8 can be considered as a prognostic biomarker for patients with luminal BC. DUXAP8, LINC00963, and FOXD2-AS1 are involved in several cancer-associated signaling pathways and multiple cancer-related processes. In addition, bioinformatic analyses indicated that LINC00963/hsa-mir-130a-3p/HSPA8 axis might have potential regulatory role in BC. In conclusion, dysregulation of DUXAP8, LINC00963, and FOXD2-AS1 can play roles in the development of luminal BC. They may exert their functions through involvement in some cancer signaling pathways and processes. In addition, they may interact with miRNAs like predicted interaction of LINC00963 with miR-130a-3p.

大量研究表明，lncRNAs参与了不同癌症的调控网络。据报道，在各种癌症的发展中，各种 lncRNA 的失调，如 DUXAP8、LINC00963 和 FOXD2-AS1。本研究的目的是调查 DUXAP8、LINC00963 和 FOXD2-AS1 在 ER ⁺中的重要性和潜在作用乳腺癌（BC）。我们通过 qRT-PCR 检测与相邻的非肿瘤组织和 MCF10A 细胞系相比，检测了 71 个管腔 A 和 B 肿瘤组织以及两种管腔 A 细胞系（MCF7 和 T47D）中 DUXAP8、LINC00963 和 FOXD2-AS1 的表达水平，分别。为了确定三种 lncRNA 与 luminal BC 之间的关系，使用一些数据库和软件进行生物信息学分析，包括 GENEVESTIGATOR 软件、GEPIA2、DAVID、REVIGO、STRING、lncATLAS、Kaplan-Meier plotter、starBase 和 miRNet 工具。结果显示管腔 A 和 B 肿瘤标本和细胞系中所有三种 lncRNA 均显着上调。DUXAP8 和 FOXD2-AS1 的上调与孕激素受体阳性（PR ⁺) 和 p53 蛋白在管腔 BC 患者中的表达。基于生物信息学分析，DUXAP8 可被视为管腔 BC 患者的预后生物标志物。DUXAP8、LINC00963 和 FOXD2-AS1 参与多种癌症相关信号通路和多种癌症相关过程。此外，生物信息学分析表明 LINC00963/hsa-mir-130a-3p/HSPA8 轴可能在 BC 中具有潜在的调节作用。总之，DUXAP8、LINC00963 和 FOXD2-AS1 的失调可以在管腔 BC 的发展中发挥作用。它们可能通过参与某些癌症信号通路和过程来发挥其功能。此外，它们可能与 miRNA 相互作用，如预测的 LINC00963 与 miR-130a-3p 的相互作用。