Insect pest control is facing a major challenge due to the increase of insecticide resistance, so alternative approaches are urgently needed. In this present study, selenium nanoparticles (SeNPs) were synthesized using Trichoderma fungus culture filtrate and tested on Spodoptera litura larvae. Synthesized SeNPs was characterized using UV–Vis, XRD, FTIR, SEM, EDaX, and DLS analysis. UV–Vis spectroscopy shows a maximum peak at 259 nm. The presence of multiple functional groups such as, anhydrides, phosphines, nitro groups, alkanes, alcohols and sulfonates was identified by using FTIR. Synthesized SeNPs are spherical in shape as per SEM analysis. EDaX analysis confirms the presence of selenium elemental compound (87.98%). XRD study reveals that the selenium appears as crystalline in nature. DLS shows that SeNPs size range between 40 to 100 nm. SeNPs tested for larvicidal activity on S. litura larvae at three different concentrations showed significant larval mortality after 48 h of exposure LC₅₀ and LC₉₀ value of SeNPs were 39.739 and 142.839 ppm respectively. Highest antifeedant activity was observed in 100 μg/ml at 48 h of SeNPs exposure. The overall results shows that the Trichoderma, selenium nanoparticles are very effective as larvicidal and antifeedant agent and can be used for control of S. litura larvae.

由于增加了对杀虫剂的抗性，害虫的防治面临着重大挑战，因此迫切需要替代方法。在本研究中，使用木霉菌培养滤液合成硒纳米颗粒（SeNPs），并在斜纹夜蛾上进行了测试幼虫。使用UV-Vis，XRD，FTIR，SEM，EDaX和DLS分析对合成的SeNPs进行表征。紫外可见光谱在259 nm处显示最大峰。使用FTIR鉴定了多个官能团（例如酸酐，膦，硝基，烷烃，醇和磺酸盐）的存在。根据SEM分析，合成的SeNP呈球形。EDaX分析证实了硒元素化合物的存在（87.98％）。XRD研究表明，硒在自然界中以结晶形式出现。DLS显示，SeNP的大小范围在40到100 nm之间。在暴露于48小时的LC ₅₀和LC _90下，在三种不同浓度下对斜纹夜蛾幼虫进行杀幼虫活性测试的SeNPs均显示出显着的幼虫死亡率。SeNPs的值分别为39.739 ppm和142.839 ppm。在SeNPs暴露48 h时，以100μg/ ml观察到最高的拒食活性。总体结果表明，木霉属硒纳米粒作为杀幼虫剂和拒食剂非常有效，可用于防治斜纹夜蛾幼虫。