The present study describes two full-genome sequences of Felis catus papillomavirus type 4 (FcaPV4) identified in squamous cell carcinoma (SCC) of two domestic cats. Two full-genome sequences of FcaPV4 were detected and characterized by PCR and sequencing. The L1 nucleotide sequence homology of one case showed 95.70% sequence identity to the reference FcaPV4, suggesting that this isolate should be classified as a subtype. Reverse-transcriptase PCR (RT-PCR) of two oncogenes, E6 and E7 was performed to confirm mRNA expression. Expression of E6 and E7 mRNA was detected in both cases, suggesting that FcaPV4 contributes to the development of SCC. This is the first report of FcaPV4 subtype. The present study will update the genomic features of FcaPV4 and contribute to deepening our knowledge about the etiological roles of FcaPV4 in feline cutaneous SCC.

本研究描述了Felis catus 的两个全基因组序列在两只家猫的鳞状细胞癌 (SCC) 中鉴定出 4 型乳头瘤病毒 (FcaPV4)。通过PCR和测序检测并表征了FcaPV4的两个全基因组序列。一个病例的 L1 核苷酸序列同源性显示与参考 FcaPV4 有 95.70% 的序列同一性，表明该分离株应归类为一个亚型。对两种癌基因 E6 和 E7 进行逆转录酶 PCR (RT-PCR) 以确认 mRNA 表达。在两种情况下都检测到 E6 和 E7 mRNA 的表达，表明 FcaPV4 有助于 SCC 的发展。这是 FcaPV4 亚型的第一份报告。本研究将更新 FcaPV4 的基因组特征，并有助于加深我们对 FcaPV4 在猫皮肤 SCC 中的病因学作用的认识。