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Full-genome characterization of a novel Felis catus papillomavirus 4 subtype identified in a cutaneous squamous cell carcinoma of a domestic cat

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Abstract

The present study describes two full-genome sequences of Felis catus papillomavirus type 4 (FcaPV4) identified in squamous cell carcinoma (SCC) of two domestic cats. Two full-genome sequences of FcaPV4 were detected and characterized by PCR and sequencing. The L1 nucleotide sequence homology of one case showed 95.70% sequence identity to the reference FcaPV4, suggesting that this isolate should be classified as a subtype. Reverse-transcriptase PCR (RT-PCR) of two oncogenes, E6 and E7 was performed to confirm mRNA expression. Expression of E6 and E7 mRNA was detected in both cases, suggesting that FcaPV4 contributes to the development of SCC. This is the first report of FcaPV4 subtype. The present study will update the genomic features of FcaPV4 and contribute to deepening our knowledge about the etiological roles of FcaPV4 in feline cutaneous SCC.

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Authors and Affiliations

  1. Division of Infection Control and Disease Prevention, Department of Veterinary Medical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

    Nanako Yamashita-Kawanishi, Yuuka Gushino & Takeshi Haga

  2. School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan, ROC

    Chia-Yu Chang & Hui-Wen Chang

  3. Laboratory of Veterinary Pathology, Department of Veterinary Medical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

    James K. Chambers & Kazuyuki Uchida

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  1. Nanako Yamashita-Kawanishi
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  2. Yuuka Gushino
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  3. Chia-Yu Chang
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  4. Hui-Wen Chang
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  5. James K. Chambers
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  6. Kazuyuki Uchida
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  7. Takeshi Haga
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Contributions

TH and HWC conceived the study; NYK, YG, and CYC performed the experiments; NYK, YG, and CYC analyzed the data; JKC and KU provided the samples and contributed to histopathological analysis; NYK wrote the manuscript. All authors revised and approved the final manuscript.

Corresponding author

Correspondence to Takeshi Haga.

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Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors. Sample materials were derived from the Laboratory of Veterinary Pathology in the University of Tokyo, submitted for diagnostic purpose.

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Informed consent is not required because no human participants were involved in this article.

Additional information

Edited by Juergen A Richt.

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Supplementary Information

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11262_2021_1843_MOESM1_ESM.pdf

Supplementary file1 (PDF 549 kb) Supplementary Figure 1 FcaPV4 E6 and E7 mRNA expression by reverse-transcriptase PCR (RT-PCR). Gel electrophoresis results confirmed cDNA (mRNA) expression of FcaPV4 E6 (top) and E7 (bottom) by RT-PCR in isolates 13-136 and 14-1110. A single band around the expected size of 158 bp (E6) and 139 bp (E7) was confirmed by RT-PCR in both cases. Abbreviations: bp, base pair; DNased, DNase-treated.

11262_2021_1843_MOESM2_ESM.xlsx

Supplementary file2 (XLSX 13 kb) Supplementary Table 1 Primers used for PCR, reverse-transcriptase PCR, and sequencing in this study. The nucleotide location and the expected amplicon size refer to the reference FcaPV4 (GenBank accession number: KF147892). Abbreviation: bp, base pairs; F, forward; nt, nucleotide; R, reverse; RT-PCR, reverse-transcriptase PCR.

11262_2021_1843_MOESM3_ESM.xlsx

Supplementary file3 (XLSX 13 kb) Supplementary Table 2 Nucleotide and amino acid substitutions observed in isolates 13-136 and 14-1110. For ORF-coding regions, the nucleotide substitutions which result in missense mutations are indicated. Nucleotide substitutions observed in the LCR and intergenic region (3836-4120nt) are noted. The nucleotide and amino acid positions refer to the reference FcaPV4 (GenBank accession number: KF147892). Abbreviation: nt, nucleotide; ORF, open reading frame.

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Yamashita-Kawanishi, N., Gushino, Y., Chang, CY. et al. Full-genome characterization of a novel Felis catus papillomavirus 4 subtype identified in a cutaneous squamous cell carcinoma of a domestic cat. Virus Genes 57, 380–384 (2021). https://doi.org/10.1007/s11262-021-01843-y

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