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Exogenous EBL (24-Epibrassinolide) Alleviate Cold Damage in Strawberry
Erwerbs-Obstbau ( IF 1.2 ) Pub Date : 2021-05-20 , DOI: 10.1007/s10341-021-00566-6
Gülden Balcı , Servet Aras , Hakan Keles

Low temperature constitutes one of the major threats in horticulture and is adversely affecting fruit production worldwide. Plant hormones, including 24-Epibrassinolide (EBL), may mitigate the malignant effects of cold stress on plants. We investigated the influences of EBL application on protein and proline content, lipid peroxidation and organic and phenolic acids of strawberry plant under cold stress. Two days before the chilling stress, three different EBL doses (1, 2 and 4 mM) were treated once (in August) to plant rhizoplane (above plant parts of soil) as solution except control. To perform chilling treatment, the plants were transferred to a cold storage from the greenhouse. Storage temperature decreased from 15 °C to 7 °C step by step and plants were hold 24 h at 7 °C. End of the study, 4 mM EBL decreased protein content in leaf and root, while it increased in stem. Among tissues of strawberry plant, higher accumulation of malondialdehyde (MDA) was observed in leaves, therefore it can be accepted that leaves are more vulnerable than stems and roots. Different EBL doses enhanced phenolic acids compared to control. The predominant phenolic acid was chlorogenic acid. EBL application increased organic acids except oxalic and malic acids compared to control. The main organic acid found in strawberry leaves exposed to cold stress in the current experiment was malic acid followed by citric and tartaric acids. Our results demonstrated that EBL alleviated the deleterious effects of cold stress in strawberry plant may be due to organic and phenolic acids and maintaining cell membrane integrity.



中文翻译:

外源EBL(24-表油菜素内酯)减轻草莓的冷害

低温是园艺的主要威胁之一,并且对全世界的水果生产产生不利影响。植物激素,包括24-表油菜素内酯(EBL),可能减轻冷胁迫对植物的恶性作用。我们研究了EBL施用对冷胁迫下草莓植株蛋白质和脯氨酸含量,脂质过氧化以及有机酸和酚酸的影响。在低温胁迫前两天,除对照外,分别对三种不同的EBL剂量(1、2和4 mM)进行了处理(八月),以种植根际平面(土壤上方的植物部分)作为溶液。为了进行冷藏处理,将植物从温室转移到冷藏库中。储存温度逐步从15°C降至7°C,植物在7°C下保持24小时。研究结束时,4 mM EBL降低了叶和根中的蛋白质含量,而它的茎增加了。在草莓植物的组织中,观察到叶片中丙二醛(MDA)的积累更高,因此可以接受的是叶片比茎和根更脆弱。与对照相比,不同的EBL剂量可增强酚酸。主要的酚酸是绿原酸。与对照相比,EBL施用增加了除草酸和苹果酸以外的有机酸。在当前实验中,暴露于冷胁迫下的草莓叶片中发现的主要有机酸是苹果酸,其次是柠檬酸和酒石酸。我们的结果表明,EBL减轻了草莓植物冷胁迫的有害影响,可能是由于有机酸和酚酸并维持了细胞膜的完整性。叶片中丙二醛(MDA)的积累更高,因此可以接受的是叶片比茎和根更脆弱。与对照相比,不同的EBL剂量可增强酚酸。主要的酚酸是绿原酸。与对照相比,EBL施用增加了除草酸和苹果酸以外的有机酸。在当前实验中,暴露于冷胁迫下的草莓叶片中发现的主要有机酸是苹果酸,其次是柠檬酸和酒石酸。我们的结果表明,EBL减轻了草莓植物冷胁迫的有害影响,可能是由于有机酸和酚酸并维持了细胞膜的完整性。叶片中丙二醛(MDA)的积累更高,因此可以接受的是叶片比茎和根更脆弱。与对照相比,不同的EBL剂量可增强酚酸。主要的酚酸是绿原酸。与对照相比,EBL施用增加了除草酸和苹果酸以外的有机酸。在当前实验中,暴露于冷胁迫下的草莓叶片中发现的主要有机酸是苹果酸,其次是柠檬酸和酒石酸。我们的结果表明,EBL减轻了草莓植物冷胁迫的有害影响,可能是由于有机酸和酚酸并维持了细胞膜的完整性。与对照相比,不同的EBL剂量可增强酚酸。主要的酚酸是绿原酸。与对照相比,EBL施用增加了除草酸和苹果酸以外的有机酸。在当前实验中,暴露于冷胁迫下的草莓叶片中发现的主要有机酸是苹果酸,其次是柠檬酸和酒石酸。我们的结果表明,EBL减轻了草莓植物冷胁迫的有害影响,可能是由于有机酸和酚酸并维持了细胞膜的完整性。与对照相比,不同的EBL剂量可增强酚酸。主要的酚酸是绿原酸。与对照相比,EBL施用增加了除草酸和苹果酸以外的有机酸。在当前实验中,暴露于冷胁迫下的草莓叶片中发现的主要有机酸是苹果酸,其次是柠檬酸和酒石酸。我们的结果表明,EBL减轻了草莓植物冷胁迫的有害影响,可能是由于有机酸和酚酸并维持了细胞膜的完整性。

更新日期:2021-05-20
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