Thirteen out of the 15 known Cryobacterium spp. were from extremely cold environments. However, the fundamental question on their cold adaptation strategies to survive in the cold has not been addressed adequately. Hence, this work was conducted to determine the Cryobacterium sp. SO1 cold adaptation strategies. Cryobacterium sp. SO1 that grew optimally at 20 °C was exposed to a sub-optimal temperature of 10 °C. Its mRNA was extracted, sequenced, and analyzed. Strain SO1 global transcriptional profiles revealed a total of 182 differential expressed genes. Four hydrolases, a clp protease, and novel YraN family endonuclease that were related to the programmed cell death pathway were upregulated, indicating that the temperature drop was probably lethal to some cells. Three highly upregulated transcriptional regulators were likely to be the key components to regulate genome-wide expression to adapt to the cold. Meanwhile, the oligo-ribonuclease and a Clp protease gene were upregulated probably to remove accumulated misfolded mRNA and proteins, respectively. The SerB gene was upregulated probably to provide more L-serine residue for the biosynthesis of cold-adapted proteins. Interestingly, most of the stress protein genes in the genome, such as the reactive oxygen species (ROS)-scavenging enzymes were not upregulated. Instead, strain SO1 upregulated the six ribosomal genes which were the target of oxidative nucleobase damage caused by the ROS. This mechanism was probably to ensure that the protein biosynthesis machinery was not affected. Overall, strain SO1 had all the necessary genes and well-coordinated mechanisms to adapt to the sub-optimal growth temperature.

在15种已知的低温细菌中，有13种。来自极端寒冷的环境。但是，关于它们在寒冷中生存的寒冷适应策略的基本问题尚未得到充分解决。因此，进行了这项工作以确定低温细菌。SO1冷适应策略。低温细菌sp。在20°C下最佳生长的SO1暴露于10°C的次优温度。提取，测序和分析其mRNA。SO1菌株的全球转录谱显示共有182个差异表达基因。与程序性细胞死亡途径有关的四种水解酶，一种clp蛋白酶和新型YraN家族核酸内切酶被上调，表明温度下降可能对某些细胞具有致死性。三个高度上调的转录调节因子可能是调节全基因组表达以适应寒冷的关键成分。同时，寡核糖核酸酶和Clp蛋白酶基因可能被上调，以分别去除累积的错误折叠的mRNA和蛋白质。在塞尔维亚该基因可能被上调，以提供更多的L-丝氨酸残基，用于冷适应蛋白质的生物合成。有趣的是，基因组中的大多数应激蛋白基因，例如清除活性氧（ROS）的酶均未上调。相反，菌株SO1上调了6个核糖体基因，这6个核糖体基因是由ROS引起的氧化核碱基损伤的靶标。该机制可能是为了确保蛋白质生物合成机制不受影响。总的来说，SO1菌株具有所有必要的基因和协调良好的机制，以适应次优的生长温度。