The development of rearing protocols promoting the larval development, pre and post-metamorphosis are key for echinoculture. Mixed diets combining diatom with other microalgae have been used with success and Rhodomonas sp. (Rho)-based diets showed promising results in larval survival. This study was aimed to evaluate the rearing success of Paracentrotus lividus larvae fed with mixed diets combining Rho with two diatoms, Phaedactylum tricornutum (Phae) and Chaetoceros calcitrans (Chae) in two experiments. In experiment I, the effect of the mixed diet of Rho and Phae (Mix I) was compared with monospecific diets of both species, while in experiment II, Rho was combined with Chae (Mix II) and compared with both monospecific diets. In experiment I, larvae fed with Rho I and Mix I diets grew faster than larvae fed with Phae I diet, attained the competence earlier with survival rates of 15-16%. In experiment II, the larvae fed with Rho were larger and the larvae fed with Mix II attained the highest survival rate (32.22%). The analysis of the larval biometric models showed that the larger larvae, with bigger stomachs, and shorter post-oral arm attained the age-at-competence earlier with higher survival rates. In conclusion, the larvae fed with a combination of Rho with the selected diatoms showed identical growth performance and condition to larvae fed with Rho monospecific diet but obtained higher survival rate. These results indicate that mixed-diatoms diets may be more suitable for P. lividus larval rearing.

养育方案的发展促进幼虫的发育，变态前和变态后是棘皮动物养殖的关键。将硅藻与其他微藻混合使用的混合饮食已获得成功，红景天菌也已成功使用。基于（Rho）的饮食在幼虫存活方面显示出令人鼓舞的结果。这项研究旨在评估混合Rho和两种硅藻Phaedactylum tricornutum（Phae）和Chaetoceros calcitrans混合日粮喂养的Paracentrotus lividus幼虫的饲养成功（蔡）在两个实验中。在实验I中，将Rho和Phae（Mix I）混合饮食与两种物种的单特异性饮食的效果进行了比较，而在实验II中，Rho与Chae（Mix II）混合使用，并与两种单特异性饮食进行了比较。在实验一中，饲喂Rho I和Mix I饲料的幼虫比饲喂Phae I饲料的幼虫生长更快，其成年率达到15-16％。在实验II中，喂食Rho的幼虫更大，喂食Mix II的幼虫存活率最高（32.22％）。幼虫生物特征模型的分析表明，较大的幼虫，较大的胃和较短的口腔后臂可以达到较早的成活年龄，并具有较高的存活率。综上所述，用Rho和选定的硅藻混合喂养的幼虫显示出与用Rho单特异性饮食喂养的幼虫相同的生长性能和条件，但获得了更高的成活率。这些结果表明混合硅藻饮食可能更适合丁香假单胞菌幼虫饲养。