The objective of the present study was to investigate the impact of iron deficiency and iron replenishment on serum iron (Fe), copper (Cu), manganese (Mn), and zinc (Zn) speciation and tissue accumulation in a deferrioxamine-induced model of iron deficiency. A total of 26 male Wistar rats were divided into three groups: control; Fe-deficient; Fe-replenished (with iron (II) gluconate). Serum ferritin and transferrin levels were assessed using immunoturbudimetric method. Liver, spleen, and serum metal levels were assessed using ICP-MS. Speciation analysis was performed using a hyphenated HPLC-ICP-MS technique. Desferrioxamine injections resulted in a significant decrease in tissue iron content that was reversed by Fe supplementation. Iron speciation revealed a significant increase in serum transferrin-bound iron and reduced ferritin-bound Fe levels. Serum but not tissue Cu levels were characterized by a significant decrease in hypoferremic rats, whereas ceruloplasmin-bound fraction tended to increase. At the same time, Zn levels were found to be higher in liver, spleen, and serum of Fe-deficient rats with a predominant increase in low molecular weight fraction.Both iron-deficient and iron-replenished rats were characteirzed by increased transferrin-bound Mn levels and reduced low-molecular weight fraction. Hypothetically, these differences may be associated with impaired Fe metabolism under Fe-deficient conditions predisposing to impairment of essential metal handling. However, further studies aimed at assessment of the impact on Fe deficiency on metal metabolism are highly required.

本研究的目的是研究铁缺乏和铁补充对去铁胺诱导模型中血清铁 (Fe)、铜 (Cu)、锰 (Mn) 和锌 (Zn) 形态和组织积累的影响。缺铁。将26只雄性Wistar大鼠分为三组：对照组；缺铁；补充铁（用葡萄糖酸铁（II））。使用免疫比浊法评估血清铁蛋白和转铁蛋白水平。使用 ICP-MS 评估肝脏、脾脏和血清金属水平。使用联用 HPLC-ICP-MS 技术进行形态分析。去铁胺注射导致组织铁含量显着降低，而补充铁可逆转这一趋势。铁形态显示血清转铁蛋白结合铁显着增加，铁蛋白结合铁水平降低。血清但不是组织中的铜水平的特点是低铁大鼠显着降低，而铜蓝蛋白结合部分则趋于增加。同时，发现缺铁大鼠肝脏、脾脏和血清中的锌水平较高，其中低分子量部分主要增加。缺铁和补铁大鼠的特征是转铁蛋白结合增加Mn 水平和降低的低分子量部分。假设，这些差异可能与缺铁条件下的铁代谢受损有关，从而导致基本金属处理受损。然而，进一步的研究旨在评估缺铁对金属代谢的影响是非常必要的。而铜蓝蛋白结合部分趋于增加。同时，发现缺铁大鼠肝脏、脾脏和血清中的锌水平较高，其中低分子量部分主要增加。缺铁和补铁大鼠的特征是转铁蛋白结合增加Mn 水平和降低的低分子量部分。假设，这些差异可能与缺铁条件下的铁代谢受损有关，从而导致基本金属处理受损。然而，进一步的研究旨在评估缺铁对金属代谢的影响是非常必要的。而铜蓝蛋白结合部分趋于增加。同时，发现缺铁大鼠肝脏、脾脏和血清中的锌水平较高，其中低分子量部分主要增加。缺铁和补铁大鼠的特征是转铁蛋白结合增加Mn 水平和降低的低分子量部分。假设，这些差异可能与缺铁条件下的铁代谢受损有关，从而导致基本金属处理受损。然而，进一步的研究旨在评估缺铁对金属代谢的影响是非常必要的。缺铁和补铁大鼠的特征是与转铁蛋白结合的锰水平增加和低分子量部分减少。假设，这些差异可能与缺铁条件下的铁代谢受损有关，从而导致基本金属处理受损。然而，进一步的研究旨在评估缺铁对金属代谢的影响是非常必要的。缺铁和补铁大鼠的特征是与转铁蛋白结合的锰水平增加和低分子量部分减少。假设，这些差异可能与缺铁条件下的铁代谢受损有关，从而导致基本金属处理受损。然而，进一步的研究旨在评估缺铁对金属代谢的影响是非常必要的。