Abstract
The objective of the present study was to investigate the impact of iron deficiency and iron replenishment on serum iron (Fe), copper (Cu), manganese (Mn), and zinc (Zn) speciation and tissue accumulation in a deferrioxamine-induced model of iron deficiency. A total of 26 male Wistar rats were divided into three groups: control; Fe-deficient; Fe-replenished (with iron (II) gluconate). Serum ferritin and transferrin levels were assessed using immunoturbudimetric method. Liver, spleen, and serum metal levels were assessed using ICP-MS. Speciation analysis was performed using a hyphenated HPLC-ICP-MS technique. Desferrioxamine injections resulted in a significant decrease in tissue iron content that was reversed by Fe supplementation. Iron speciation revealed a significant increase in serum transferrin-bound iron and reduced ferritin-bound Fe levels. Serum but not tissue Cu levels were characterized by a significant decrease in hypoferremic rats, whereas ceruloplasmin-bound fraction tended to increase. At the same time, Zn levels were found to be higher in liver, spleen, and serum of Fe-deficient rats with a predominant increase in low molecular weight fraction.Both iron-deficient and iron-replenished rats were characteirzed by increased transferrin-bound Mn levels and reduced low-molecular weight fraction. Hypothetically, these differences may be associated with impaired Fe metabolism under Fe-deficient conditions predisposing to impairment of essential metal handling. However, further studies aimed at assessment of the impact on Fe deficiency on metal metabolism are highly required.
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The manuscript prepared with support of The Foundation for Assistance to Small Innovative Enterprises within the Project No. 379GRNTIS5/42620 from 23.08.2018 (0042620) Application No. NTI-48849.
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The study was funded by Foundation for Assistance to Small Innovative Enterprises in Science and Technology (Grant No. 379GRNTIS5/42620).
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10534_2021_318_MOESM1_ESM.tif
Figure S1. SEC-ICP-MS chromatograms of used standards: A - SEC-ICP-MS chromatograms of the iron blood serum species (red curve) and calibration standards (black curve – ferritin; blue curve – transferrin; green curve – iron (III) citrate, yellow - albumine). Preparation of metal complexes with chosen ligands was performed according to section ‘2.4. Speciation analysis of Fe, Zn, Cu, Mn in blood serum’. The peak corresponding to albumin is lower, because the complex of multimetal standard with albumin gave a pronounced signal in the case of copper, but not in the iron case. To our opinion this fact can be explained the high binding affinity of Cu and the low binding affinity of Fe. B – SEC-ICP-MS chromatograms for an albumin standard containing Cu and Fe at the same concentrations. The plot was obtained as a result of overlaying of the individual chromatograms. Supplementary file 1 (TIF 287 kb)
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Ajsuvakova, O.P., Skalnaya, M.G., Michalke, B. et al. Alteration of iron (Fe), copper (Cu), zinc (Zn), and manganese (Mn) tissue levels and speciation in rats with desferioxamine-induced iron deficiency. Biometals 34, 923–936 (2021). https://doi.org/10.1007/s10534-021-00318-9
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DOI: https://doi.org/10.1007/s10534-021-00318-9