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Influence of different types of explants in chickpea regeneration using thidiazuron seed-priming
Journal of Plant Research ( IF 2.7 ) Pub Date : 2021-05-15 , DOI: 10.1007/s10265-021-01312-5
Pragati Kumari 1 , Sumer Singh 1 , Saurabh Yadav 2 , Lam-Son Phan Tran 3, 4
Affiliation  

A comparative regeneration of three types of explants prepared from axillary meristems, plumular apices and hypocotyls of chickpea (Cicer arietinum) was carried out using four thidiazuron (TDZ) treatment methods. The first and third ones included the short-term 20 μM TDZ pre-treatment for all three explant types followed by non-supplementation or supplementation of TDZ (4 μM) into the shoot induction medium (SIM), while the second and fourth ones lacked TDZ pre-treatment followed by non-addition or addition of 4 μM TDZ in the SIM. Axillary meristem explants produced the best results with seed pre-treatment using 20 μM TDZ without TDZ in SIM and showed the highest rate of regeneration efficiency (71.33 ± 1.5%) after 20 days. Concurrently, plumular apex explants from TDZ-primed seeds was ranked second, exhibiting a regeneration percentage of 54.33 ± 2.3% in SIM without supplementation of TDZ, whereas explants from hypocotyls generated from seeds subjected to any of the TDZ treatments were not regenerated on any SIMs after 20 days.



中文翻译:

不同类型外植体对噻唑隆种子引发鹰嘴豆再生的影响

三种类型从腋生分生组织,plumular顶点和鹰嘴豆的下胚轴外植体来制备的的比较再生(鹰嘴豆) 使用四种噻唑脲 (TDZ) 处理方法进行。第一个和第三个包括对所有三种外植体类型进行短期 20 μM TDZ 预处理,然后在芽诱导培养基 (SIM) 中不补充或补充 TDZ (4 μM),而第二个和第四个则缺乏TDZ 预处理,然后在 SIM 中不添加或添加 4 μM TDZ。腋生分生组织外植体在 SIM 中使用 20 μM TDZ 进行种子预处理时产生最佳结果,并在 20 天后显示出最高的再生效率 (71.33 ± 1.5%)。同时,来自 TDZ 引发种子的羽状先端外植体排名第二,在 SIM 中未补充 TDZ 的再生百分比为 54.33 ± 2.3%,

更新日期:2021-05-15
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