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Influence of different types of explants in chickpea regeneration using thidiazuron seed-priming

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Abstract

A comparative regeneration of three types of explants prepared from axillary meristems, plumular apices and hypocotyls of chickpea (Cicer arietinum) was carried out using four thidiazuron (TDZ) treatment methods. The first and third ones included the short-term 20 μM TDZ pre-treatment for all three explant types followed by non-supplementation or supplementation of TDZ (4 μM) into the shoot induction medium (SIM), while the second and fourth ones lacked TDZ pre-treatment followed by non-addition or addition of 4 μM TDZ in the SIM. Axillary meristem explants produced the best results with seed pre-treatment using 20 μM TDZ without TDZ in SIM and showed the highest rate of regeneration efficiency (71.33 ± 1.5%) after 20 days. Concurrently, plumular apex explants from TDZ-primed seeds was ranked second, exhibiting a regeneration percentage of 54.33 ± 2.3% in SIM without supplementation of TDZ, whereas explants from hypocotyls generated from seeds subjected to any of the TDZ treatments were not regenerated on any SIMs after 20 days.

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Acknowledgements

The authors are thankful to the Agriculture Research Station, Durgapura, Jaipur, India for the supply of chickpea varieties. We are thankful to Dr. Hemant R. Kushwaha, School of Biotechnology, Jawaharlal Nehru University, India, for helping in statistical analysis.

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Authors and Affiliations

  1. Department of Life Sciences, Singhania University, Jhunjhunu, Rajasthan, 333515, India

    Pragati Kumari & Sumer Singh

  2. Department of Biotechnology, Hemvati Nandan Bahuguna Garhwal (Central) University, Srinagar Garhwal, Uttarakhand, 246174, India

    Saurabh Yadav

  3. Institute of Research and Development, Duy Tan University, 03 Quang Trung, Da Nang, Vietnam

    Lam-Son Phan Tran

  4. Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX, 79409, USA

    Lam-Son Phan Tran

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  1. Pragati Kumari
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  2. Sumer Singh
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  3. Saurabh Yadav
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  4. Lam-Son Phan Tran
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Correspondence to Saurabh Yadav or Lam-Son Phan Tran.

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Kumari, P., Singh, S., Yadav, S. et al. Influence of different types of explants in chickpea regeneration using thidiazuron seed-priming. J Plant Res 134, 1149–1154 (2021). https://doi.org/10.1007/s10265-021-01312-5

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