The unbalance of reactive oxygen species (ROS) has been associated with the exacerbation of possible diseases, such as cancer and neurodegenerative illnesses. Mushrooms are very rich in bioactive nutrients such as phenolic compounds, polysaccharides, vitamins and other compounds that have antioxidant potential. A substrate obtained during mushrooms development is of great interest in terms of biomass valorization, since it can be reused for energy production. The aim of this study was to evaluate the effect of different concentrations, 0.1, 0.5 and 1 g/L of phenolic compounds, extracted from the Tricholoma equestre mushroom species, produced from biomass, in the formation of cellular reactive oxygen species. The studies were performed in brain slices, using the fluorescent ROS indicator H₂DCFDA. The results indicate that, for all concentrations, the mushroom phenolics enhanced the neuronal ROS signals, which include autofluorescence, in a concentration dependent way. The data obtained in the presence of the synthetic phenolic, 4-hydroxybenzoic acid, which is the most abundant phenolic in the Tricholoma mushrooms, are similar to those found for the same concentration (1 g/L) of the phenolic extracts. However, upon removal, while some of the signals evoked by the extracts were not reversible those induced by the synthetic compound fully recovered. The equivalent antioxidant capacity of the Tricholoma phenolics was determined using the TEAC and the DPPH techniques. The analyses revealed that these phenolics have a large antioxidant capacity.

Graphic Abstract

中文翻译：

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木质纤维素生物质产生的蘑菇酚类化合物引起的Ros信号

活性氧（ROS）的不平衡与诸如癌症和神经退行性疾病等可能疾病的恶化有关。蘑菇中的生物活性营养非常丰富，例如酚类化合物，多糖，维生素和其他具有抗氧化潜力的化合物。在蘑菇开发过程中获得的基质在生物质增值方面非常受关注，因为它可以重新用于能源生产。这项研究的目的是评估从生物量产生的马口蘑菌种中提取的0.1、0.5和1 g / L的不同浓度的酚类化合物对细胞活性氧物质形成的影响。使用荧光ROS指示剂H ₂在脑切片中进行研究DCFDA。结果表明，在所有浓度下，香菇酚类药物均以浓度依赖性方式增强了神经元ROS信号，包括自发荧光。在口蘑中蘑菇中酚含量最高的合成酚4-羟基苯甲酸的存在下获得的数据与相同浓度（1 g / L）酚提取物的数据相似。然而，在去除时，虽然提取物引起的一些信号是不可逆的，但由合成化合物完全回收的那些信号却是不可逆的。使用TEAC和DPPH技术确定了口蘑酚醛树脂的等效抗氧化能力。分析表明，这些酚类物质具有很大的抗氧化能力。

图形摘要