Abstract
The unbalance of reactive oxygen species (ROS) has been associated with the exacerbation of possible diseases, such as cancer and neurodegenerative illnesses. Mushrooms are very rich in bioactive nutrients such as phenolic compounds, polysaccharides, vitamins and other compounds that have antioxidant potential. A substrate obtained during mushrooms development is of great interest in terms of biomass valorization, since it can be reused for energy production. The aim of this study was to evaluate the effect of different concentrations, 0.1, 0.5 and 1 g/L of phenolic compounds, extracted from the Tricholoma equestre mushroom species, produced from biomass, in the formation of cellular reactive oxygen species. The studies were performed in brain slices, using the fluorescent ROS indicator H2DCFDA. The results indicate that, for all concentrations, the mushroom phenolics enhanced the neuronal ROS signals, which include autofluorescence, in a concentration dependent way. The data obtained in the presence of the synthetic phenolic, 4-hydroxybenzoic acid, which is the most abundant phenolic in the Tricholoma mushrooms, are similar to those found for the same concentration (1 g/L) of the phenolic extracts. However, upon removal, while some of the signals evoked by the extracts were not reversible those induced by the synthetic compound fully recovered. The equivalent antioxidant capacity of the Tricholoma phenolics was determined using the TEAC and the DPPH techniques. The analyses revealed that these phenolics have a large antioxidant capacity.
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References
Birben, E., Sahiner, U.M., Sackesen, C., Erzurum, S., Kalayci, O.: Oxidative stress and antioxidant defense. World Allergy Organiz. J. 5(1), 9–19 (2012). https://doi.org/10.1097/WOX.0b013e3182439613
Kozarski, M., Klaus, A., Jakovljevic, D., Todorovic, N., Vunduk, J., Petrović, P., Niksic, M., Vrvic, M.M., Griensven, L.V.: Antioxidants of edible mushrooms. Molecules 20, 19489–19525 (2015). https://doi.org/10.3390/molecules201019489
Ribeiro, B., Rangel, J., Valentão, P., Baptista, P., Seabra, R.M., Andrade, P.B.: Contents of carboxylic acids and two phenolics and antioxidant activity of dried portuguese wild edible mushrooms. J. Agricul. Food Chem. 54(22), 8530–8537 (2006). https://doi.org/10.1021/jf061890q
Lemieszek, M.K., Nunes, F.M., Cardoso, C., Marques, G., Rzeski, W.: Neuroprotective properties of Cantharellus cibarius polysaccharide fractions in different in vitro models of neurodegeneration. Carbohyd. Polym. 197, 598–607 (2018). https://doi.org/10.1016/j.carbpol.2018.06.038
Reynerston, K.A., Yang, H., Jiang, B., Basile, M.J., Kennelly, E.J.: Quantitative analysis of antiradical phenolic constituents from fourteen edible Myrtaceae fruits. Food Chem. 109(4), 883–890 (2008). https://doi.org/10.1016/j.foodchem.2008.01.021
Kim, M.Y., Seguin, P., Ahn, J.K., Kim, J.J., Chun, S.C., Kim, E.H., Seo, S.H., Kang, E.Y., Kim, S.L., Park, Y.J., Ro, H.M., Chung, I.M.: Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J. Agricul. Food Chem. 56, 7265–7270 (2008). https://doi.org/10.1021/jf8008553
Korkmaz, S.: Antioxidants in Maca (Lepidium meyenii) as a supplement in nutrition. Antioxidants Foods Its Appl (2018). https://doi.org/10.5772/intechopen.75582
Smina, T.P., Mathew, J., Janardhanan, K.K., Devasagayam, T.P.A.: Antioxidant activity and toxicity profile of total triterpenes isolated from Ganoderma lucidum (Fr.) P. Karst occurring in South India. Environ. Toxicol. Pharmacol. 32, 438–446 (2011). https://doi.org/10.1016/j.etap.2011.08.011
Rouches, E., Herpoël-Gimbert, I., Steyer, J.P., Carrere, H.: Improvement of anaerobic degradation by white-rot fungi pretreatment of lignocellulosic biomass: a review. Renew. Sustain. Energy Rev. 59, 179–198 (2015). https://doi.org/10.1016/j.rser.2015.12.317
Wang, Y., Xu, B.: Distribution of antioxidant activities and total phenolic contents in acetone, ethanol, water and hot water extracts from 20 edible mushrooms via sequential extraction. Austin J. Nutr. Food Sci. 2(1), 1009 (2014)
Alves, M.J., Ferreira, I.C.F.R., Froufe, H.J.C., Abreu, R.M.V., Martins, A., Pintado, M.: Antimicrobial activity of phenolic compounds identified in wild mushrooms SAR analysis and docking studies. J. Appl. Microbiol. 115(2), 346–357 (2013). https://doi.org/10.1111/jam.12196
Robaszkiewicz, A., Bartosz, G., Ławrynowicz, M. and Soszynski, M. (2010). The role of polyphenols, β-carotene, and lycopene in the antioxidative action of the extracts of dried, edible mushrooms. J. Nutr. Metab. https://doi.org/https://doi.org/10.1155/2010/173274.
Bilski, P., Li, M.Y., Ehrenshaft, M., Daub, M.E., Chignell, C.F.: Vitamin B6 (pyridoxine) and its derivatives are efficient singlet oxygen quenchers and potential fungal antioxidants. Photochem. Photobiol. 71(2), 129–134 (2000). https://doi.org/10.1562/0031-8655(2000)0710129SIPVBP2.0.CO2
Wei, S., Helsper, J., Leonardus, J., Lambertus, D., Van, G.: Phenolic compounds present in medicinal mushroom extracts generate reactive oxygen species in human cells in vitro. Int. J. Med. Mushrooms 10(1), 1–13 (2008). https://doi.org/10.1615/IntJMedMushr.v10.i1
Acknowledgements
We thank CNC—Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal, for providing the rat brains. Work funded by strategic project UID/NEU/04539/2013.
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Miranda, C.F., Pessoa, A., Batista, M. et al. Ros Signals Induced by Mushrooms Phenolic Compounds Produced from Lignocellulosic Biomass. Waste Biomass Valor 12, 3027–3033 (2021). https://doi.org/10.1007/s12649-021-01385-2
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DOI: https://doi.org/10.1007/s12649-021-01385-2