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Screening of newly isolated marine-derived fungi for their laccase production and decolorization of different dye types
Regional Studies in Marine Science ( IF 2.1 ) Pub Date : 2021-05-12 , DOI: 10.1016/j.rsma.2021.101837
Sultan Kübra Toker , Hüseyin Evlat , Ali Koçyi̇ği̇t

Six marine-derived fungi (Alternaria sp. D11, Alternaria sp. D21, Cadophora sp. D43, Cadophora luteo-olivacea D51, Phoma sp. K2BR, Phoma sp. K21) were isolated from the Çakalburnu Lagoon, Izmir Bay, Aegean Sea in Turkey. An effective method has been developed for marine-derived fungi isolation and laccase production of the fungi has been studied. This method consisted of immersing a sterile solid substrate containing lignin into the aquatic system under aseptic conditions and removing at the end of 10 days, isolation for laccase producing fungi of marine origin. The decolorization potential of the isolate Alternaria sp. D21 with the highest laccase activity was investigated for dyes containing azo, anthraquinone, triarylmethane and heterocyclic chemical classes. The decolorization rates were determined as 98% for Methyl orange, 93% for Acid green 3, 78% for Methylene blue, 71% for Remazol Brilliant Blue, 58% for Crystal violet, and 53% for Congo red after 144 h at 100 mg/L concentration of dyes. The ability of the marine-derived fungi to remove dyes of different chemical classes is also promising for waste containing mixtures of various pollutants.



中文翻译:

筛选新分离的海洋来源真菌的漆酶生产和不同染料类型的脱色

在爱琴海的Çakalburnu泻湖,伊兹密尔湾,爱琴海中分离出六种海洋来源的真菌(链格孢属D11,链格孢属D21,Cadophora属D43,Cadophora luteo-olivacea D51,Phoma属K2BR,Phoma属K21)。火鸡。已经开发出一种有效的方法来分离海洋来源的真菌,并且已经研究了真菌的漆酶生产。该方法包括在无菌条件下将无菌的含有木质素的固体基质浸入水生系统中,并在10天后去除,分离出产生漆酶的海洋来源真菌。分离链霉菌的脱色潜力sp。研究了具有最高漆酶活性的D21,用于含有偶氮,蒽醌,三芳基甲烷和杂环化学类别的染料。在100 mg剂量下放置144 h后,甲基橙的脱色率为98%,酸性绿3为93%,亚甲基蓝为78%,Remazol亮蓝为71%,结晶紫为58%,刚果红为53%。 / L染料浓度。对于含有多种污染物混合物的废物,海洋真菌去除各种化学类别染料的能力也是有希望的。

更新日期:2021-05-25
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