Packed bed bioreactors were used to remove soluble manganese from a synthetic mine water as the final stage of an integrated bioremediation process. The synthetic mine water had undergone initial processing using a sulfidogenic bioreactor (pH 4.0–5.5) which removed all transition metals present in elevated concentrations (Cu, Ni, Zn and Co) apart from manganese. The aerobic bioreactors were packed with pebbles collected from a freshwater stream that were coated with black-colored, Mn(IV)-containing biofilms, and their capacity to remove soluble Mn (II) from the synthetic mine water was tested at varying hydraulic retention times (11–45 h) and influent liquor pH values (5.0 or 6.5). Over 99% of manganese was removed from the partly processed mine water when operated at pH 6.5 and a HRT of 45 h. Molecular techniques (clone libraries and T-RFLP analysis) were used to characterize the biofilms and identified two heterotrophic Mn-oxidizing microorganisms: the bacterium Leptothrix discophora and what appears to be a novel fungal species. The latter was isolated and characterized in vitro.

中文翻译：

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经验性脱锰生物反应器的设计和运行，并集成到用于从酸性矿井水中修复和回收金属的复合模块化系统中

填充床生物反应器用于从合成矿井水中去除可溶性锰，这是综合生物修复工艺的最后阶段。合成矿井水已使用硫化生物反应器（pH 4.0-5.5）进行了初步处理，该反应器除去了锰以外所有浓度较高的过渡金属（铜，镍，锌和钴）。好氧生物反应器装有从淡水流中收集的卵石，卵石上覆盖有黑色的含Mn（IV）的生物膜，并在不同的水力停留时间下测试了它们从合成矿井水中去除可溶性Mn（II）的能力。 （11–45小时）和进水pH值（5.0或6.5）。在pH 6.5和HRT为45 h的条件下运行时，从部分处理的矿井水中去除了超过99％的锰。Leptothrix  discophora和似乎是一个新的真菌物种。后者被分离并在体外表征。