Soil bacteria play important roles in regulating biogeochemical cycles and maintaining ecosystem function. However, our knowledge of patterns and driving factors of permafrost microbial communities under typical alpine meadow habitats with different vegetation communities is limited. We analyzed the relationship between soil bacterial community and changes of environmental factors among four habitats (swamp wetland, swamp meadow, meadow, and mature meadow) with different dominant vegetation types on the eastern edge of Qinghai-Tibetan, China. The results showed that though the bacterial community composition among four habitats was similar, the proportions of dominant species and the bacterial biomarkers in each habitat were unique. The bacterial community structure of mature meadow was obviously distinct from the other three habitats, which derived from the significant difference in soil nutrients and shoot biomass compared to the others. Specifically, distance-based redundancy analysis showed that soil total nitrogen and soil water content explained the greatest difference in bacterial structures, followed by soil temperature, pH and shoot biomass. Different habitats formed various network patterns, which showed more significant connections in swamp meadow and meadow than swamp wetland and mature meadow. Rhizomicrobium, Pseudonocardia, Geobacter, Bryobacter, Syntrophorhabdus, Nitrospira, Solirubrobacter and Pedomicrobium were the most important keystone genera in the bacterial co-occurrence networks.

土壤细菌在调节生物地球化学循环和维持生态系统功能中起着重要作用。但是，我们对具有不同植被群落的典型高寒草甸生境下多年冻土微生物群落的模式和驱动因素的了解是有限的。我们分析了青藏高原东部边缘不同优势植被类型的四个生境（沼泽湿地，沼泽草甸，草甸和成熟草甸）中土壤细菌群落与环境因子变化之间的关系。结果表明，尽管四个生境之间的细菌群落组成相似，但每个生境中优势种的比例和细菌生物标志物都是独特的。成熟草甸的细菌群落结构明显不同于其他三个生境，这是由于土壤养分和枝条生物量与其他养分相比存在显着差异。具体而言，基于距离的冗余分析表明，土壤总氮和土壤水分解释了细菌结构的最大差异，其次是土壤温度，pH和枝条生物量。不同的生境形成了不同的网络格局，与沼泽湿地和成熟草甸相比，沼泽草甸和草甸之间的联系更为明显。Rhizomicrobium，假，地杆菌，Bryobacter，Syntrophorhabdus，硝化，Solirubrobacter和Pedomicrobium是在细菌共生网络中最重要的基石属。