New symptoms including aborted seed pods disease were observed on mungbean (Vigna radiata) plants in the most important growing areas in Iran that was similar to symptoms of phytoplasma disease previously reported from Australia. Thirty samples were collected from symptomatic and non-symptomatic plants, followed by PCR amplification, DNA sequencing, RFLP and phylogenetic analysis of 16S ribosomal RNA gene to detect and identify possible associated phytoplasmas. Results indicated that two different phytoplasmas, related to ‘Candidatus Phytoplasma trifolii’ and ‘Candidatus Phytoplasma aurantifolia’, were associated with different symptomatic plants samples from the same field, mixed infection was not observed in these samples. The sequence analysis results indicated that the nucleotide sequences from mungbean isolates 47 (MT674290), 7 (MT674291) and 32 (MT674292) had 98.8% sequence identity to ‘Candidatus Phytoplasma aurantifolia’ isolate TBBP-H1 (Acc. No: MN565885), while isolates 80 (MT674293) and 25 (MT674294) had 99.8% sequence identity similar to ‘Candidatus Phytoplasma trifolii’ (MF092789). In silico RFLP and phylogenetic analyse of mungbean seed pod abortion (MubSpa) phytoplasma indicated that the associated phytoplasmas belonged to phytoplasmas of subgroups 16SrVI-A and 16SrII-D. To the best of our knowledge, this is the first report of association ‘Ca. P. trifolii’ and ‘Ca. P. aurantifolia’ strains infecting mungbean plants in Iran.

在伊朗最重要的产区， 在绿豆（Vigna radiata）植物上观察到了包括中止种子荚病在内的新症状，这种症状类似于以前从澳大利亚报道的植物胞浆病的症状。从有症状和无症状植物中收集了三十份样品，然后进行PCR扩增，DNA测序，RFLP和16S核糖体RNA基因的系统发育分析，以检测和鉴定可能的相关植物质体。结果表明，两种不同的植物质原体分别与“ Candidatus Phytoplasma trifolii”和“ Candidatus”有关。植原体与来自同一领域的不同有症状植物样品相关，在这些样品中未观察到混合感染。序列分析结果表明，从绿豆分离株47的核苷酸序列（MT674290），7（MT674291）和32（MT674292）具有98.8％的序列同一性'暂定植原体aurantifolia'分离TBBP-H1（Acc编号：MN565885），而分离物80（MT674293）和25（MT674294）具有类似于'99.8％的序列同一性暂定Trifolii trifolii'（MF092789）。在计算机RFLP上和对绿豆种子荚流产（MubSpa）植物原质的系统发育分析表明，相关的植物原质属于亚组16SrVI-A和16SrII-D的植物质。据我们所知，这是“ Ca ”协会的第一份报告。P. trifolii'和' Ca. Aurantifolia的菌株感染伊朗的绿豆植物。