Senescence is the phenomenon by which physiological functions of organisms degenerate with time. Cellular senescence is marked by an inhibition of cell cycle progression. Beta-galactosidase accumulates in the lysosomes of aged cells. In this study, human dermal fibroblast cells (HDFs) were treated with 0.5 μM doxorubicin for 4 h to induce cellular senescence. Senescence-associated beta-galactosidase (SA-β-gal) activity was then measured 72 h after treatment with aerial parts of Dendranthema zawadskii var. lucidum (Nakai) J.H. Park (DZ) extract. Treatment with DZ extract significantly decreased SA-β-gal activity in a dose-dependent manner in HDFs. Additionally, DZ extract treatment reduced age-related oxidative stress and inflammation in the aortas of aged rats. The reactive oxygen species (ROS) levels in aortas of aged control rats were higher than those in young rats. However, DZ extract-fed aged rats showed significantly lower ROS levels than the aged control rats. When the aged rats were treated with DZ extract at either 0.2 or 1.0 mg∙kg⁻¹∙day⁻¹, NF-κB levels in aorta tissue decreased significantly compared to those in aorta tissue of the aged control rats without DZ treatment. In addition, DZ extract-fed aged rat aortas showed significant reductions in expression of iNOS and COX-2 induced by NF-κB translocation. Therefore, these results suggest that DZ effectively inhibited senescence-related NF-κB activation and inflammation. DZ extract may have a role in the prevention of the vascular inflammatory responses that occur during vascular aging.

中文翻译：

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Dendranthema zawadskii var。灵芝（Nakai）JH Park提取物抑制人皮肤成纤维细胞的细胞衰老和大鼠衰老相关的炎症

衰老是生物体的生理功能随着时间而退化的现象。细胞衰老的特征在于细胞周期进程的抑制。β-半乳糖苷酶在衰老细胞的溶酶体中积累。在这项研究中，人类皮肤成纤维细胞（HDF）用0.5μM阿霉素处理4小时，以诱导细胞衰老。然后在用Dendranthema zawadskii var的空中部分处理72小时后，测量与衰老相关的β-半乳糖苷酶（SA-β-gal）活性。灵芝（中井）JH Park（DZ）提取物。用DZ提取物处理可显着降低HDF中SA-β-gal的活性，并呈剂量依赖性。此外，DZ提取物治疗可减少老年大鼠主动脉中与年龄相关的氧化应激和炎症。老年对照组大鼠的主动脉中的活性氧（ROS）水平高于年轻大鼠。但是，DZ提取物喂养的老年大鼠显示的ROS水平显着低于老年对照大鼠。当老年大鼠用0.2或1.0 mg∙kg ^-1 ∙day ^-1的DZ提取物治疗时与未进行DZ治疗的老年对照组大鼠的主动脉组织相比，主动脉组织中的NF-κB水平显着降低。此外，DZ提取物喂养的老年大鼠主动脉显示出由NF-κB易位引起的iNOS和COX-2表达的显着降低。因此，这些结果表明DZ有效地抑制了衰老相关的NF-κB的活化和炎症。DZ提取物可能在预防血管衰老过程中发生的血管炎症反应中起作用。