Metal mining extraction and concentration need water. The recycling of liquid effluents reduces its consumption. This recycled water must contain low concentrations of metals. This project’s first objective was to start up a laboratory-scale upflow anaerobic sludge blanket (UASB) reactor for biotransforming metal sulfates into metal sulfides of effluent from flotation units of a mining plant (FE). The second objective was to determine the effect of pH and chemical oxygen demand:sulfate ratio (COD:SO₄²⁻) in the precipitation of heavy metal sulfides. The third objective, which is the subject of this part of the research, was to evaluate the feasibility of this proposal through a mass balance of the metals separated by precipitation through the formation of sulfides by sulfate-reducing microorganisms (SRM), using as energy source carbonaceous compounds from flotation residual organic reagents enriched with lactic acid. To monitor bioconversion effectiveness, various parameters were used: pH-alkalinity factor (α), temperature (T), COD, SO₄²⁻, and sulfides (S²⁻). Four metals were considered for this part of the research: Cu, Pb, Zn, and Fe. The UASB system achieved a sulfate bioconversion of 69% and an organic matter removal as COD of 88% after transient state with the best COD:SO₄²⁻ ratio found. Using a statistical analysis by clusters, metal sulfide production of 74 mg L⁻¹ and removals of 39, 70, 79, and 65%, for Pb, Cu Zn, and Fe, respectively, were obtained. These values were calculated with an initial ratio of COD:SO₄²⁻ of 0.66 ± 0.2 and pH values around 6 inside the reactor, through anaerobic microbial biomass, indicating that the solution proposed to recycle water in the metal extraction and concentration is feasible after using these systems.

金属采矿的提取和浓缩需要水。废液的循环利用减少了其消耗。这种循环水必须包含低浓度的金属。该项目的第一个目标是启动一个实验室规模的上流厌氧污泥床（UASB）反应器，以将金属硫酸盐生物转化为采矿厂（FE）浮选装置产生的废水中的金属硫化物。第二个目标是确定pH值和化学需氧量：硫酸盐比率（COD：SO ₄ ^2-）中重金属硫化物的沉淀。第三部分是本部分研究的主题，其目的是通过利用硫酸盐还原微生物（SRM）形成硫化物形成沉淀物，以能量作为能量，通过沉淀分离出的金属的质量平衡来评估该建议的可行性。从浮选的富含乳酸的残留有机试剂中提取碳质化合物。为了监测生物转化的有效性，使用了各种参数：pH碱度因子（α），温度（T），COD，SO ₄ ^2-和硫化物（S ^2-）。本部分研究考虑了四种金属：Cu，Pb，Zn和Fe。UASB系统在瞬态后以最佳的COD：SO ₄ ^2-比率实现了69％的硫酸盐生物转化率和88％的有机物去除率。通过聚类的统计分析，分别获得了Pb，Cu Zn和Fe的金属硫化物产量为74 mg L ^-1，去除率分别为39、70、79和65％。通过厌氧微生物生物质，以COD：SO ₄ ^2-的初始比率为0.66±0.2和pH值约为6的方式计算出这些值，表明拟议中的循环水用于金属萃取和浓缩后的溶液是可行的。使用这些系统。