The Botryosphaeriaceae is a family of endophytic fungi, many of which are latent pathogens of woody plants. Although extensively sampled in some parts of the world, little is known regarding their occurrence across different environmental conditions. This study considered the presence of the Botryosphaeriaceae on Syzygium cordatum trees across a latitudinal gradient. We examined the relative importance of different environmental factors on the presence of the Botryosphaeriaceae across this latitudinal gradient. Specifically, Botryosphaeriaceae community composition and species richness were analysed. The optimal growth temperature of the most common Botryosphaeriaceae isolates and its relation to isolate origin was also tested in culture. We identified 14 Botryosphaeriaceae species including seven each of Lasiodiplodia and Neofusicoccum species. The maximum historical temperature emerged as the environmental factor that best predicted the presence of Botryosphaeriaceae species in S. cordatum trees, specifically influencing Botryosphaeriaceae community composition. For all the Botryosphaeriaceae species studied in vitro, temperature strongly influenced mycelial growth and they all had an optimal growth temperature of 25 °C. Contrary to our hypothesis, the optimal growth temperature was not related to isolate origin. These results contribute to understanding the presence of the Botryosphaeriaceae in trees and our ability to detect these latent pathogens.

Botryosphaeriaceae 是一个内生真菌家族，其中许多是木本植物的潜在病原体。尽管在世界某些地区进行了广泛采样，但对其在不同环境条件下的发生情况知之甚少。该研究考虑了葡萄球菌科在横贯纬度梯度的Syzygium cordatum树上的存在。我们研究了不同环境因素对葡萄球菌科在这个纬度梯度上的相对重要性。具体来说，分析了葡萄球菌群落组成和物种丰富度。最常见的葡萄球菌科分离物的最佳生长温度及其与分离物来源的关系也在培养中进行了测试。我们鉴定了 14 种葡萄球菌科物种，其中每一种都有 7 种Lasiodiplodia和Neofusicoccum物种。最高历史温度成为最能预测S.cordatum树中葡萄球菌科物种存在的环境因素，特别是影响葡萄球菌科群落组成。对于体外研究的所有葡萄球菌科物种，温度强烈影响菌丝体生长，它们的最佳生长温度均为 25 °C。与我们的假设相反，最佳生长温度与分离物来源无关。这些结果有助于了解树木中 Botryosphaeriaceae 的存在以及我们检测这些潜在病原体的能力。