Species of Piperaceae are known by biological properties, including antiparasitic such as leishmanicidal, antimalarial and in the treatment of schistosomiasis. The aim of this work was to evaluate the antileishmania activity, cytotoxic effect, and macrophage activation patterns of the methanol (MeOH), hexane (HEX), dichloromethane (DCM) and ethyl acetate (EtOAc) extract fractions from the leaves of Piper cabralanum C.DC. The MeOH, HEX and DCM fractions inhibited Leishmanina amazonensis promastigote-like forms growth with a half maximal inhibitory concentration (IC 50 ) of 144.54, 59.92, and 64.87 μg/mL, respectively. The EtOAc fraction did not show any relevant activity. The half maximal cytotoxic concentration (CC 50 ) for macrophages were determined as 370.70, 83.99, 113.68 and 607 μg/mL for the MeOH, HEX and DCM fractions, respectively. The macrophage infectivity was concentration-dependent, especially for HEX and DCM. MeOH, HEX and DCM fractions showed activity against L. amazonensis with low cytotoxicity to murine macrophages and lowering infectivity by the parasite. Our results provide support for in vivo studies related to a potential application of P. cabralanum extract and fractions as a promising natural resource in the treatment of leishmaniasis.

中文翻译：

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Piper cabralanum C.DC 提取物的抗利什曼原虫活性。（胡椒科）

胡椒科物种的生物学特性包括抗寄生虫药，如杀利什曼原虫、抗疟药和治疗血吸虫病。这项工作的目的是评估来自 Piper cabralanum C 叶子的甲醇 (MeOH)、己烷 (HEX)、二氯甲烷 (DCM) 和乙酸乙酯 (EtOAc) 提取物组分的抗利什曼原虫活性、细胞毒性作用和巨噬细胞活化模式。 .DC。MeOH、HEX 和 DCM 组分抑制 Leishmanina amazonensis 前鞭毛体样形式的生长，其半数最大抑制浓度 (IC 50) 分别为 144.54、59.92 和 64.87 μg/mL。EtOAc 馏分未显示任何相关活性。对于 MeOH、HEX 和 DCM 级分，巨噬细胞的半数最大细胞毒性浓度 (CC 50) 分别测定为 370.70、83.99、113.68 和 607 μg/mL。巨噬细胞的感染性是浓度依赖性的，尤其是对于 HEX 和 DCM。MeOH、HEX 和 DCM 组分显示出针对亚马逊乳杆菌的活性，对小鼠巨噬细胞的细胞毒性较低，并降低了寄生虫的传染性。我们的结果为与 P. cabralanum 提取物和组分作为治疗利什曼病的有前途的自然资源的潜在应用相关的体内研究提供了支持。