Enzymatic hydrolysis of pre-treated wheat straw has been studied. Several additives have been checked as potential improvers of glucose yield in the enzymatic hydrolysis, trying to avoid the cellulases inhibition by the lignin as a cause of the decrease of the saccharification rate of lignocellulosic biomass. Non-ionic surfactants, such as Tween 80 (poly(oxyethylene)₂₀-sorbitan-monooleate) and polyethylene glycol-6000, and proteins, as bovine serum albumin, casein and skimmed milk powder, have been used to improve the sugars yield in the enzymatic hydrolysis. Two pre-treated wheat straw materials, one with high delignification and another with low delignification, have been tested. Three concentrations of each additive have been considered. The results obtained point out that the lignin content of the pre-treated lignocellulosic biomass has a decisive influence in reaching a high yield in the enzymatic saccharification. The additives checked have a moderate effect on the cellulose hydrolysis rate. The more favorable option combines high delignification with the use of a cheap protein such as casein, reaching a glucose yield of 88.6% when the best conditions are employed.

已经研究了预处理的麦秸的酶水解。已经检查了几种添加剂作为酶促水解中葡萄糖产量的潜在改进剂，试图避免木质素对纤维素酶的抑制，这是木质纤维素生物质糖化率降低的原因。非离子表面活性剂，例如Tween 80（聚（氧乙烯）₂₀-脱水山梨糖醇-单油酸酯和聚乙二醇-6000，以及蛋白质，如牛血清白蛋白，酪蛋白和脱脂奶粉，已被用于改善酶解过程中糖的收率。已经测试了两种预处理过的麦秸材料，一种具有高去木质素度，另一种具有低去木质素度。已经考虑了每种添加剂的三种浓度。所获得的结果指出，预处理的木质纤维素生物质中木质素含量对酶促糖化中达到高产率具有决定性的影响。检查的添加剂对纤维素水解速率有中等影响。更有利的选择是将高去木质素与廉价蛋白质（如酪蛋白）结合使用，当采用最佳条件时，可达到88.6％的葡萄糖产率。